Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult Schistosoma mansoni Using DIG-Labeled RNA Probes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F20%3AN0000145" target="_blank" >RIV/60460709:41210/20:N0000145 - isvavai.cz</a>

Výsledek na webu

<a href="https://experiments.springernature.com/articles/10.1007/978-1-0716-0635-3_4" target="_blank" >https://experiments.springernature.com/articles/10.1007/978-1-0716-0635-3_4</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult Schistosoma mansoni Using DIG-Labeled RNA Probes

Popis výsledku v původním jazyce

In situ hybridization is a tool for evaluation of gene expression within tissues or single cells. This protocol describes optimized sensitive fluorescence detection of gene transcripts in semithin sections of Schistosoma mansoni adult worms using specifically designed and labeled RNA probes. Due to improved methodologies in tissue preservation, sectioning, amplification of fluorescent signal, and prehybridization tissue treatment, it is possible to detect transcripts in the fine structures of schistosomes. The protocol is sensitive enough to detect very low abundance targets. This procedure is optimized for tissues derived from S. mansoni adult worms, however, it can be successfully applied to other trematode species. Methods In Molecular Biology, Schistosoma mansoni Methods and Protocols. Humana Press, Springer Science. Totowa, New Jersey, United States. p265. ISBN 978-1-0716-0635-3. DOI 10.1007/978-1-0716-0635-3_4

Název v anglickém jazyce

Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult Schistosoma mansoni Using DIG-Labeled RNA Probes

Popis výsledku anglicky

In situ hybridization is a tool for evaluation of gene expression within tissues or single cells. This protocol describes optimized sensitive fluorescence detection of gene transcripts in semithin sections of Schistosoma mansoni adult worms using specifically designed and labeled RNA probes. Due to improved methodologies in tissue preservation, sectioning, amplification of fluorescent signal, and prehybridization tissue treatment, it is possible to detect transcripts in the fine structures of schistosomes. The protocol is sensitive enough to detect very low abundance targets. This procedure is optimized for tissues derived from S. mansoni adult worms, however, it can be successfully applied to other trematode species. Methods In Molecular Biology, Schistosoma mansoni Methods and Protocols. Humana Press, Springer Science. Totowa, New Jersey, United States. p265. ISBN 978-1-0716-0635-3. DOI 10.1007/978-1-0716-0635-3_4

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30310 - Parasitology

Projekt

<a href="/cs/project/GA18-14167S" target="_blank" >GA18-14167S: Orthologní glutamát karboxypeptidázy 2 v modelových organizmech - hledání fyziologických rolí a terapeutického potenciálu enigmatického enzymu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů