Development of new methodologies for rapid determination of oxidizable and reducible organic compounds using batch injection analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F21%3A10442113" target="_blank" >RIV/00216208:11310/21:10442113 - isvavai.cz</a>

Výsledek na webu

<a href="http://web.natur.cuni.cz/analchem/isc/isc17.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc17.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of new methodologies for rapid determination of oxidizable and reducible organic compounds using batch injection analysis

Popis výsledku v původním jazyce

Batch injection analysis (BIA) offers many advantages, e.g., rapid analysis, high reproducibility, sufficient selectivity, and minimal sample and reagent consumption. The development of a new method using BIA with amperometric detection (BIA-AD) for sensitive determination of chlorpromazine is described in this contribution. The following parameters were optimized within the method development: pH of media (supporting electrolyte), detection potential, rate of dispense, and volume of dispense. Under the optimized conditions (pH = 6, detection potential of 700 mV, dispense rate of 286.3 μL s, injected volume of 50 μL), a linear calibration dependence of chlorpromazine was measured at a screen-printed carbon electrode in the concentration range 1-1000 μmol L, with a limit of quantification 2.7 μmol L. Applicability of the newly develo-ped method was verified on determination of chlorpromazine in pharmaceutical formulation Plegomazine with satisfactory results. It is worth mentioning that air oxygen needs to be removed in BIA-AD from analyzed solutions while reducible compounds are determined. One possible solution to overcome this complication is shown in experiments with metronidazole, where adding solid sodium sulfite as a reductive agent shows comparable results to the traditional oxygen removal with nitrogen gas.

Název v anglickém jazyce

Development of new methodologies for rapid determination of oxidizable and reducible organic compounds using batch injection analysis

Popis výsledku anglicky

Batch injection analysis (BIA) offers many advantages, e.g., rapid analysis, high reproducibility, sufficient selectivity, and minimal sample and reagent consumption. The development of a new method using BIA with amperometric detection (BIA-AD) for sensitive determination of chlorpromazine is described in this contribution. The following parameters were optimized within the method development: pH of media (supporting electrolyte), detection potential, rate of dispense, and volume of dispense. Under the optimized conditions (pH = 6, detection potential of 700 mV, dispense rate of 286.3 μL s, injected volume of 50 μL), a linear calibration dependence of chlorpromazine was measured at a screen-printed carbon electrode in the concentration range 1-1000 μmol L, with a limit of quantification 2.7 μmol L. Applicability of the newly develo-ped method was verified on determination of chlorpromazine in pharmaceutical formulation Plegomazine with satisfactory results. It is worth mentioning that air oxygen needs to be removed in BIA-AD from analyzed solutions while reducible compounds are determined. One possible solution to overcome this complication is shown in experiments with metronidazole, where adding solid sodium sulfite as a reductive agent shows comparable results to the traditional oxygen removal with nitrogen gas.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GC20-01417J" target="_blank" >GC20-01417J: Nové přístupy zabraňující pasivaci elektrod při monitorování environmentálních organických polutantů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 17th International Students Conference &quot;Modern Analytical Chemistry&quot;

ISBN

978-80-7444-089-2

ISSN

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e-ISSN

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Počet stran výsledku

7

Strana od-do

66-72

Název nakladatele

Univerzita Karlova, Přírodovědecká fakulta

Místo vydání

Praha

Místo konání akce

Prague

Datum konání akce

16. 9. 2021

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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