Detection of Pyocyanin with a Boron-doped Diamond Electrode Using Flow Injection Analysis with Amperometric Detection and Square Wave Voltammetry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10435898" target="_blank" >RIV/00216208:11310/22:10435898 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=qPwhmOgxvJ" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=qPwhmOgxvJ</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elan.202100562" target="_blank" >10.1002/elan.202100562</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of Pyocyanin with a Boron-doped Diamond Electrode Using Flow Injection Analysis with Amperometric Detection and Square Wave Voltammetry

Popis výsledku v původním jazyce

We report on the analytical performance of boron-doped diamond thin-film electrodes for the detection of the virulence factor, pyocyanin (PYO). The results reported herein confirm the utility of diamond electrodes for the sensitive and reproducible detection of pyocyanin in a 0.1 mol L(-1) phosphate buffer (pH 7.4) when using amperometric detection coupled with flow injection analysis (FIA) or square wave voltammetry (SWV). Pyocyanin was reductively detected by hydrodynamic voltammetry in FIA at an E(1/2) of -0.31 V vs. Ag/AgCl (3 mol L(-1) KCl). The detection figures of merit were a linear dynamic range from 100 to 0.08 μmol L(-1), a response reproducibility of 1.3 % (RSD), and a limit of detection of 0.09 μmol L(-1) (S/N=3). A comparable linear dynamic range, response reproducibility and limit of detection were observed in preliminary square wave voltammetry measurements. Importantly, these detection figures of merit were obtained using boron-doped diamond electrodes that received no conventional pretreatment. Some comparison data are presented for nitrogen-incorporated tetrahedral amorphous carbon (ta-C:N) and glassy carbon (GC) electrodes.

Název v anglickém jazyce

Detection of Pyocyanin with a Boron-doped Diamond Electrode Using Flow Injection Analysis with Amperometric Detection and Square Wave Voltammetry

Popis výsledku anglicky

We report on the analytical performance of boron-doped diamond thin-film electrodes for the detection of the virulence factor, pyocyanin (PYO). The results reported herein confirm the utility of diamond electrodes for the sensitive and reproducible detection of pyocyanin in a 0.1 mol L(-1) phosphate buffer (pH 7.4) when using amperometric detection coupled with flow injection analysis (FIA) or square wave voltammetry (SWV). Pyocyanin was reductively detected by hydrodynamic voltammetry in FIA at an E(1/2) of -0.31 V vs. Ag/AgCl (3 mol L(-1) KCl). The detection figures of merit were a linear dynamic range from 100 to 0.08 μmol L(-1), a response reproducibility of 1.3 % (RSD), and a limit of detection of 0.09 μmol L(-1) (S/N=3). A comparable linear dynamic range, response reproducibility and limit of detection were observed in preliminary square wave voltammetry measurements. Importantly, these detection figures of merit were obtained using boron-doped diamond electrodes that received no conventional pretreatment. Some comparison data are presented for nitrogen-incorporated tetrahedral amorphous carbon (ta-C:N) and glassy carbon (GC) electrodes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

1521-4109

Svazek periodika

34

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

1902-1912

Kód UT WoS článku

000741283700001

EID výsledku v databázi Scopus

2-s2.0-85122749234