Phosphorothioate oligonucleotides separation in ion-pairing reversed-phase liquid chromatography: Effect of ion-pairing system
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10451267" target="_blank" >RIV/00216208:11310/22:10451267 - isvavai.cz</a>
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=OGUn9kOkni" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=OGUn9kOkni</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2022.463201" target="_blank" >10.1016/j.chroma.2022.463201</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Phosphorothioate oligonucleotides separation in ion-pairing reversed-phase liquid chromatography: Effect of ion-pairing system
Popis výsledku v původním jazyce
Ion-pairing reversed-phase liquid chromatography was utilized for the analysis of native and phosphorothioate oligonucleotides of the identical sequence but different amount and position of phosphorothioate modifications. The effects of ion-pairing amines nature (alkyl chain length, hydrophobicity) and concentration on retention, n / n-1 resolution, and diastereomeric separation of phosphorothioate oligonucleotides were investigated using octadecyl column. Eight different ion-pairing agents at two concentrations (10 mM and 100 mM) buffered with acetic acid were investigated. The resolution of n and n-1 mers oligonucleotides improved with hydrophobicity and concentration of ion-pairing amines. Ion-pairing amines with moderate hydrophobicity were most successful in suppressing diastereomeric resolution. However, a partial separation of phosphorothioate oligonucleotide diastereomers was observed with all ion-pairing systems, which resulted in wider peaks compared to phosphorodiester oligonucleotides of the same sequence. This phenomenon complicates the n and n-1 mers separation of oligonucleotides with high degree of phosphorothioate modifications. Separation of oligonucleotides with different number of phosphorothioate modifications was observed, which may be useful for therapeutic oligonucleotide analysis. The aim of the work was to identify the ion-pairing systems useful for chromatographic characterization of phosphorothioate oligonucleotides.
Název v anglickém jazyce
Phosphorothioate oligonucleotides separation in ion-pairing reversed-phase liquid chromatography: Effect of ion-pairing system
Popis výsledku anglicky
Ion-pairing reversed-phase liquid chromatography was utilized for the analysis of native and phosphorothioate oligonucleotides of the identical sequence but different amount and position of phosphorothioate modifications. The effects of ion-pairing amines nature (alkyl chain length, hydrophobicity) and concentration on retention, n / n-1 resolution, and diastereomeric separation of phosphorothioate oligonucleotides were investigated using octadecyl column. Eight different ion-pairing agents at two concentrations (10 mM and 100 mM) buffered with acetic acid were investigated. The resolution of n and n-1 mers oligonucleotides improved with hydrophobicity and concentration of ion-pairing amines. Ion-pairing amines with moderate hydrophobicity were most successful in suppressing diastereomeric resolution. However, a partial separation of phosphorothioate oligonucleotide diastereomers was observed with all ion-pairing systems, which resulted in wider peaks compared to phosphorodiester oligonucleotides of the same sequence. This phenomenon complicates the n and n-1 mers separation of oligonucleotides with high degree of phosphorothioate modifications. Separation of oligonucleotides with different number of phosphorothioate modifications was observed, which may be useful for therapeutic oligonucleotide analysis. The aim of the work was to identify the ion-pairing systems useful for chromatographic characterization of phosphorothioate oligonucleotides.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/GA20-19655S" target="_blank" >GA20-19655S: Strategie pro on-line štěpení proteinů s následnou separací v mix-mode chromatografii</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
1873-3778
Svazek periodika
1676
Číslo periodika v rámci svazku
August
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
8
Strana od-do
463201
Kód UT WoS článku
000827852700010
EID výsledku v databázi Scopus
2-s2.0-85132740521