Liquid chromatography and capillary electrophoresis in glycomic and glycoproteomic analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10452236" target="_blank" >RIV/00216208:11310/22:10452236 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=1e7luoN12v" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=1e7luoN12v</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-022-02938-4" target="_blank" >10.1007/s00706-022-02938-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Liquid chromatography and capillary electrophoresis in glycomic and glycoproteomic analysis

Popis výsledku v původním jazyce

Glycosylation is one of the most significant and abundant post-translational modifications in cells. Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides (glycans) conjugated to proteins. Glycomic and glycoproteomic analysis is highly challenging because of the large diversity of structures, low abundance, site-specific heterogeneity, and poor ionization efficiency of glycans and glycopeptides in mass spectrometry (MS). MS is a key tool for characterization of glycans and glycopeptides. However, MS alone does not always provide full structural and quantitative information for many reasons, and thus MS is combined with some separation technique. This review focuses on the role of separation techniques used in glycomic and glycoproteomic analyses, liquid chromatography and capillary electrophoresis. The most important separation conditions and results are presented and discussed.

Název v anglickém jazyce

Liquid chromatography and capillary electrophoresis in glycomic and glycoproteomic analysis

Popis výsledku anglicky

Glycosylation is one of the most significant and abundant post-translational modifications in cells. Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides (glycans) conjugated to proteins. Glycomic and glycoproteomic analysis is highly challenging because of the large diversity of structures, low abundance, site-specific heterogeneity, and poor ionization efficiency of glycans and glycopeptides in mass spectrometry (MS). MS is a key tool for characterization of glycans and glycopeptides. However, MS alone does not always provide full structural and quantitative information for many reasons, and thus MS is combined with some separation technique. This review focuses on the role of separation techniques used in glycomic and glycoproteomic analyses, liquid chromatography and capillary electrophoresis. The most important separation conditions and results are presented and discussed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GJ19-18005Y" target="_blank" >GJ19-18005Y: Retenční mechanismus intaktních glykopeptidů v hydrofilní interakční kapalinové chromatografii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte für Chemie - Chemical Monthly

ISSN

0026-9247

e-ISSN

1434-4475

Svazek periodika

153

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

28

Strana od-do

659-686

Kód UT WoS článku

000814062300002

EID výsledku v databázi Scopus

2-s2.0-85132151172