Role of PML nuclear bodies during mouse polyomavirus infection: PML protein isoforms and the non-canonical histone H3.3

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10454407" target="_blank" >RIV/00216208:11310/22:10454407 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.ccsss.cz/index.php/ccsss/issue/view/37" target="_blank" >http://www.ccsss.cz/index.php/ccsss/issue/view/37</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Role of PML nuclear bodies during mouse polyomavirus infection: PML protein isoforms and the non-canonical histone H3.3

Popis výsledku v původním jazyce

The possible restriction function of promyelocytic nuclear bodies (PML NBs) and their associated proteins in polyomavirus infection was studied. At first, We found that the absence of PML protein positively affects the viral early transcription, however, we found that PML isoform 2 does not play role in the restriction. The role of other isoforms is being studied. At second we followed the possible participation of proteins transiently interacting with PML NBs, chaperones HIRA and DAXX/ATRX, in the deposition of non-canonical histone H3.3 into viral minichromosomes and the functional consequences of such incorporation. Our preliminary results show that H3.3 is incorporated not only in condensed minichromosomes in virions but also accumulated at the sites of MPyV replication. DAXX was shown to be dispensable for the deposition of H3.3 in the genomes.

Název v anglickém jazyce

Role of PML nuclear bodies during mouse polyomavirus infection: PML protein isoforms and the non-canonical histone H3.3

Popis výsledku anglicky

The possible restriction function of promyelocytic nuclear bodies (PML NBs) and their associated proteins in polyomavirus infection was studied. At first, We found that the absence of PML protein positively affects the viral early transcription, however, we found that PML isoform 2 does not play role in the restriction. The role of other isoforms is being studied. At second we followed the possible participation of proteins transiently interacting with PML NBs, chaperones HIRA and DAXX/ATRX, in the deposition of non-canonical histone H3.3 into viral minichromosomes and the functional consequences of such incorporation. Our preliminary results show that H3.3 is incorporated not only in condensed minichromosomes in virions but also accumulated at the sites of MPyV replication. DAXX was shown to be dispensable for the deposition of H3.3 in the genomes.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/LX22NPO5103" target="_blank" >LX22NPO5103: Národní institut virologie a bakteriologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů