Massive intein content in Anaeramoeba reveals aspects of intein mobility in eukaryotes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10480159" target="_blank" >RIV/00216208:11310/23:10480159 - isvavai.cz</a>
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=jSNaO4uvBF" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=jSNaO4uvBF</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.2306381120" target="_blank" >10.1073/pnas.2306381120</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Massive intein content in Anaeramoeba reveals aspects of intein mobility in eukaryotes
Popis výsledku v původním jazyce
Inteins are self- splicing protein elements found in viruses and all three domains of life. How the DNA encoding these selfish elements spreads within and between genomes is poorly understood, particularly in eukaryotes where inteins are scarce. Here, we show that the nuclear genomes of three strains of Anaeramoeba encode between 45 and 103 inteins, in stark contrast to four found in the most intein-rich eukaryotic genome described previously. The Anaeramoeba inteins reside in a wide range of proteins, only some of which correspond to intein- containing proteins in other eukaryotes, prokaryotes, and viruses. Our data also suggest that viruses have contributed to the spread of inteins in Anaeramoeba and the colonization of new alleles. The persistence of Anaeramoeba inteins might be partly explained by intragenomic movement of intein- encoding regions from gene to gene. Our intein dataset greatly expands the spectrum of intein- containing proteins and provides insights into the evolution of inteins in eukaryotes.
Název v anglickém jazyce
Massive intein content in Anaeramoeba reveals aspects of intein mobility in eukaryotes
Popis výsledku anglicky
Inteins are self- splicing protein elements found in viruses and all three domains of life. How the DNA encoding these selfish elements spreads within and between genomes is poorly understood, particularly in eukaryotes where inteins are scarce. Here, we show that the nuclear genomes of three strains of Anaeramoeba encode between 45 and 103 inteins, in stark contrast to four found in the most intein-rich eukaryotic genome described previously. The Anaeramoeba inteins reside in a wide range of proteins, only some of which correspond to intein- containing proteins in other eukaryotes, prokaryotes, and viruses. Our data also suggest that viruses have contributed to the spread of inteins in Anaeramoeba and the colonization of new alleles. The persistence of Anaeramoeba inteins might be partly explained by intragenomic movement of intein- encoding regions from gene to gene. Our intein dataset greatly expands the spectrum of intein- containing proteins and provides insights into the evolution of inteins in eukaryotes.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
<a href="/cs/project/GA21-30563S" target="_blank" >GA21-30563S: Charakterizace nové hluboké linie eukaryot s plesiomorfními mitochondriemi</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
1091-6490
Svazek periodika
120
Číslo periodika v rámci svazku
49
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
9
Strana od-do
e2306381120
Kód UT WoS článku
001158695900005
EID výsledku v databázi Scopus
2-s2.0-85178212433