Prevention of antibiotic resistance by TARGEted Treatment of pneumonia in children

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F24%3A10484745" target="_blank" >RIV/00216208:11310/24:10484745 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Prevention of antibiotic resistance by TARGEted Treatment of pneumonia in children

Popis výsledku v původním jazyce

The project focused on a fast, accurate and reliable method of recognizing specific pathogens, which will be an alternative to the standard identification of the causative agent of the disease using microbiological techniques or the polymerase chain reaction. A new and revolutionary method in this research is the application of the LAMP (loop-mediated isothermal amplification) method, which is able to detect certain DNA with high specificity and speed in one test tube. LAMP does not require any complex equipment or complicated detection of the resulting products. The current standard procedure for pathogen identification takes many hours to days, while the newly developed methodology reduces the entire process to minutes. This will also contribute to the fight against resistant bacteria, because we can treat patients practically immediately and we don&apos;t have to risk the so-called &quot;blind&quot; application of antibiotics before knowing the results of identification of pathogens using current standard methods. Such a procedure does not unnecessarily expose the patient to the side effects of ineffective antibiotics or delay the start of effective treatment, thus reducing the likelihood of the spread of resistant bacteria. Taken together, the fast and reliable LAMP method was developed for resource-limited settings to identify Streptococcus pneumoniae. We validated the method on clinical samples. Similar approach was suggested to Staphylococcus aureus, Haemophilus influenzae, and Mycoplasma pneumoniae identification.

Název v anglickém jazyce

Prevention of antibiotic resistance by TARGEted Treatment of pneumonia in children

Popis výsledku anglicky

The project focused on a fast, accurate and reliable method of recognizing specific pathogens, which will be an alternative to the standard identification of the causative agent of the disease using microbiological techniques or the polymerase chain reaction. A new and revolutionary method in this research is the application of the LAMP (loop-mediated isothermal amplification) method, which is able to detect certain DNA with high specificity and speed in one test tube. LAMP does not require any complex equipment or complicated detection of the resulting products. The current standard procedure for pathogen identification takes many hours to days, while the newly developed methodology reduces the entire process to minutes. This will also contribute to the fight against resistant bacteria, because we can treat patients practically immediately and we don&apos;t have to risk the so-called &quot;blind&quot; application of antibiotics before knowing the results of identification of pathogens using current standard methods. Such a procedure does not unnecessarily expose the patient to the side effects of ineffective antibiotics or delay the start of effective treatment, thus reducing the likelihood of the spread of resistant bacteria. Taken together, the fast and reliable LAMP method was developed for resource-limited settings to identify Streptococcus pneumoniae. We validated the method on clinical samples. Similar approach was suggested to Staphylococcus aureus, Haemophilus influenzae, and Mycoplasma pneumoniae identification.

Druh

V_souhrn - Souhrnná výzkumná zpráva

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/8F20011" target="_blank" >8F20011: Prevention of antibiotic resistance by TARGEted Treatment of pneumonia in children</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.

Počet stran výsledku

12

Místo vydání

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Název nakladatele resp. objednatele

JPIAMR (Joint Programming Initiative on Antimicrobial Resistance), Swedish Research Council, Vetenskapsrådet P.O Box 1035, SE-101 38 Stockholm, Švédsko

Verze

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