Improvement of oligonucleotide separation using a repetto high-performance liquid chromatography recycling approach

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F24%3A10487992" target="_blank" >RIV/00216208:11310/24:10487992 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=d5NPZE~jnz" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=d5NPZE~jnz</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.202400252" target="_blank" >10.1002/jssc.202400252</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Improvement of oligonucleotide separation using a repetto high-performance liquid chromatography recycling approach

Popis výsledku v původním jazyce

A new approach for the improvement of separation of oligonucleotides by recycling ion-pairing chromatography is described. In the so-called repetto process, segments of separated compounds are sequentially returned to the inlet for multiple passages through the column without a need to pass a pump and with the possibility of detecting the level of separation between individual passages. Unlike in the recently described twin-column recycle approach in which eluents are repeatedly transferred between two separation columns, with the repetto method a single column is sufficient, and the detector is not exposed to high back pressure. The repetto principle was used for the separation of synthetic oligonucleotides, resulting in a multi-fold improvement in single nt resolution of long (&gt; 50 nt) synthetic oligonucleotide fragments with high gas chromatography (guanine-cytosine) content &gt; 40% and their separation from impurities of the original synthesis.

Název v anglickém jazyce

Improvement of oligonucleotide separation using a repetto high-performance liquid chromatography recycling approach

Popis výsledku anglicky

A new approach for the improvement of separation of oligonucleotides by recycling ion-pairing chromatography is described. In the so-called repetto process, segments of separated compounds are sequentially returned to the inlet for multiple passages through the column without a need to pass a pump and with the possibility of detecting the level of separation between individual passages. Unlike in the recently described twin-column recycle approach in which eluents are repeatedly transferred between two separation columns, with the repetto method a single column is sufficient, and the detector is not exposed to high back pressure. The repetto principle was used for the separation of synthetic oligonucleotides, resulting in a multi-fold improvement in single nt resolution of long (&gt; 50 nt) synthetic oligonucleotide fragments with high gas chromatography (guanine-cytosine) content &gt; 40% and their separation from impurities of the original synthesis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

1615-9314

Svazek periodika

47

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

2400252

Kód UT WoS článku

001236451000001

EID výsledku v databázi Scopus

2-s2.0-85195008322