Interaction properties and separation potential of zwitterionic stationary phases in hydrophilic interaction liquid chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F25%3A10503950" target="_blank" >RIV/00216208:11310/25:10503950 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22310/25:43932621

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=KmLye3bWkt" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=KmLye3bWkt</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jcoa.2025.100258" target="_blank" >10.1016/j.jcoa.2025.100258</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interaction properties and separation potential of zwitterionic stationary phases in hydrophilic interaction liquid chromatography

Popis výsledku v původním jazyce

Two commercially available zwitterionic columns, one diol-based column and two newly developed experimental zwitterionic columns for hydrophilic interaction liquid chromatography (HILIC) were characterized and compared in terms of their interaction abilities. The novel HILIC columns offer mixed-mode cation-exchange and anion-exchange interactions together with minor hydrophobic contribution of alkyl spacers between the units. Tanaka testing protocol, which provides complex information about column retentivity, hydrophobicity, hydrophilicity and cation/anion-exchange characters was performed. The results revealed the differences between individual zwitterionic stationary phases and clear predominance of cation-exchange character over anion-exchange character was observed for all tested columns. The measurements at different pH at various buffer salt concentrations revealed that the predominance of negative charge on the stationary phase surface occurs at pH 4.7 and higher (probably due to the dissociation of residual silanols). At lower pH (pH 3.0) the anion-exchange mechanism also applies. The cation-exchange character (applying across the whole pH range) has been further studied using other simple chromatographic tests and compared with &gt;20 other commercially available columns. The application potential of all the tested columns was verified for analysis of tryptophan and its derivatives and also for cytochrome C tryptic digests.

Název v anglickém jazyce

Interaction properties and separation potential of zwitterionic stationary phases in hydrophilic interaction liquid chromatography

Popis výsledku anglicky

Two commercially available zwitterionic columns, one diol-based column and two newly developed experimental zwitterionic columns for hydrophilic interaction liquid chromatography (HILIC) were characterized and compared in terms of their interaction abilities. The novel HILIC columns offer mixed-mode cation-exchange and anion-exchange interactions together with minor hydrophobic contribution of alkyl spacers between the units. Tanaka testing protocol, which provides complex information about column retentivity, hydrophobicity, hydrophilicity and cation/anion-exchange characters was performed. The results revealed the differences between individual zwitterionic stationary phases and clear predominance of cation-exchange character over anion-exchange character was observed for all tested columns. The measurements at different pH at various buffer salt concentrations revealed that the predominance of negative charge on the stationary phase surface occurs at pH 4.7 and higher (probably due to the dissociation of residual silanols). At lower pH (pH 3.0) the anion-exchange mechanism also applies. The cation-exchange character (applying across the whole pH range) has been further studied using other simple chromatographic tests and compared with &gt;20 other commercially available columns. The application potential of all the tested columns was verified for analysis of tryptophan and its derivatives and also for cytochrome C tryptic digests.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography Open

ISSN

2772-3917

e-ISSN

2772-3917

Svazek periodika

8

Číslo periodika v rámci svazku

November

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

100258

Kód UT WoS článku

001585453800001

EID výsledku v databázi Scopus

2-s2.0-105016122667