High-frequency sampling unveils biotic and abiotic drivers of rapid phytoplankton morphological changes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F25%3A10509182" target="_blank" >RIV/00216208:11310/25:10509182 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=YHlJl0qXrU" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=YHlJl0qXrU</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/nph.70534" target="_blank" >10.1111/nph.70534</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High-frequency sampling unveils biotic and abiotic drivers of rapid phytoplankton morphological changes

Popis výsledku v původním jazyce

Phytoplankton, as primary producers, play a key role in aquatic ecosystems. Their community turnover is shaped by morphological traits that enable adaptation to diverse abiotic and biotic factors. Yet, the temporal scale of these dynamics remains poorly understood due to limited high-frequency sampling studies.Employing DNA metabarcoding, we assessed the community composition of the phytoplankton lineage Synurales (Chrysophyceae) at 3-d intervals during 70 d at a shallow peat bog lake in the Czech Republic. The selected group possesses a variety of species-specific key morphological traits, such as cell size, coloniality, and bristle formation.Using a custom reference database of cultured species, we assigned 99.93% of eDNA reads to 74 species-level lineages with known morphological traits. Community changes in colonial species were influenced by abiotic drivers such as silica concentration and wind speed. By contrast, shifts in unicellular species communities were mainly driven by Cladocera predators, influencing the occurrence of bristle-bearing species.Changes in species composition and morphological traits occurred within days, mirroring environmental variability. Achieving such fine-scale resolution, especially for small or rare taxa, would be extremely difficult using microscopy alone. eDNA enabled high-resolution community profiling and abundance estimation, demonstrating its key role and the importance of comprehensive reference databases.

Název v anglickém jazyce

High-frequency sampling unveils biotic and abiotic drivers of rapid phytoplankton morphological changes

Popis výsledku anglicky

Phytoplankton, as primary producers, play a key role in aquatic ecosystems. Their community turnover is shaped by morphological traits that enable adaptation to diverse abiotic and biotic factors. Yet, the temporal scale of these dynamics remains poorly understood due to limited high-frequency sampling studies.Employing DNA metabarcoding, we assessed the community composition of the phytoplankton lineage Synurales (Chrysophyceae) at 3-d intervals during 70 d at a shallow peat bog lake in the Czech Republic. The selected group possesses a variety of species-specific key morphological traits, such as cell size, coloniality, and bristle formation.Using a custom reference database of cultured species, we assigned 99.93% of eDNA reads to 74 species-level lineages with known morphological traits. Community changes in colonial species were influenced by abiotic drivers such as silica concentration and wind speed. By contrast, shifts in unicellular species communities were mainly driven by Cladocera predators, influencing the occurrence of bristle-bearing species.Changes in species composition and morphological traits occurred within days, mirroring environmental variability. Achieving such fine-scale resolution, especially for small or rare taxa, would be extremely difficult using microscopy alone. eDNA enabled high-resolution community profiling and abundance estimation, demonstrating its key role and the importance of comprehensive reference databases.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/GA23-06881S" target="_blank" >GA23-06881S: Genetická podstata speciace protist</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

New Phytologist

ISSN

0028-646X

e-ISSN

1469-8137

Svazek periodika

248

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

2528-2541

Kód UT WoS článku

001563898900001

EID výsledku v databázi Scopus

2-s2.0-105015484121