Personal Glucose Meter: Biosensing Platforms for Environmental Toxicants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F25%3A10515695" target="_blank" >RIV/00216208:11310/25:10515695 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=rPzvZzkchU" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=rPzvZzkchU</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/bios15120811" target="_blank" >10.3390/bios15120811</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Personal Glucose Meter: Biosensing Platforms for Environmental Toxicants

Popis výsledku v původním jazyce

The detection of environmental toxicants is transitioning from centralized laboratory methods to decentralized, point-of-care (POC) monitoring. A highly innovative approach in this field is the repurposing of commercially available, low-cost, and portable personal glucose meters (PGMs) as universal biosensing platforms. This strategy leverages the widespread availability and ease of use of PGMs to develop rapid, on-site detection methods for a wide array of non-glucose targets, significantly reducing both cost and development time. This systematic review comprehensively examines the various strategies employed to adapt PGMs for the detection of a wide array of ecotoxicants, including chemical targets (antibiotics, mycotoxins, pesticides, heavy metals, persistent organic pollutants) and biological ones (pathogenic bacteria, and viruses). The systematic review critically evaluates different sensor designs, highlighting that while aptamer-based and non-enzymatic biosensors offer advantages in stability and cost, antibody-based sensors provide high specificity. A significant finding is the persistent trade-off between analytical sensitivity and practical field deployment; many of the most sensitive assays require multi-step procedures, precise temperature control, magnetic separation, centrifugation, and the use of additional equipment, factors that undermine true POC utility. To address this gap, we propose four essential criteria for POC readiness: (i) ambient-temperature operation, (ii) no reliance on magnetic or centrifugal separation, (iii) total assay time, and (iv) robustness in complex environmental matrices. This systematic review confirms the feasibility of this approach across a broad spectrum of targets. However, the key challenge for future research lies in simplifying the assay protocols, eliminating cumbersome sample preparation steps, and enhancing robustness to make these biosensors truly practical for routine, on-site environmental monitoring.

Název v anglickém jazyce

Personal Glucose Meter: Biosensing Platforms for Environmental Toxicants

Popis výsledku anglicky

The detection of environmental toxicants is transitioning from centralized laboratory methods to decentralized, point-of-care (POC) monitoring. A highly innovative approach in this field is the repurposing of commercially available, low-cost, and portable personal glucose meters (PGMs) as universal biosensing platforms. This strategy leverages the widespread availability and ease of use of PGMs to develop rapid, on-site detection methods for a wide array of non-glucose targets, significantly reducing both cost and development time. This systematic review comprehensively examines the various strategies employed to adapt PGMs for the detection of a wide array of ecotoxicants, including chemical targets (antibiotics, mycotoxins, pesticides, heavy metals, persistent organic pollutants) and biological ones (pathogenic bacteria, and viruses). The systematic review critically evaluates different sensor designs, highlighting that while aptamer-based and non-enzymatic biosensors offer advantages in stability and cost, antibody-based sensors provide high specificity. A significant finding is the persistent trade-off between analytical sensitivity and practical field deployment; many of the most sensitive assays require multi-step procedures, precise temperature control, magnetic separation, centrifugation, and the use of additional equipment, factors that undermine true POC utility. To address this gap, we propose four essential criteria for POC readiness: (i) ambient-temperature operation, (ii) no reliance on magnetic or centrifugal separation, (iii) total assay time, and (iv) robustness in complex environmental matrices. This systematic review confirms the feasibility of this approach across a broad spectrum of targets. However, the key challenge for future research lies in simplifying the assay protocols, eliminating cumbersome sample preparation steps, and enhancing robustness to make these biosensors truly practical for routine, on-site environmental monitoring.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biosensors

ISSN

2079-6374

e-ISSN

2079-6374

Svazek periodika

15

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

52

Strana od-do

811

Kód UT WoS článku

001646850400001

EID výsledku v databázi Scopus

2-s2.0-105025741180