Na+,K+/H+ antiporter Nha1 ovlivňuje plazmatický membránový potenciál u Saccharomyces cerevisiae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F06%3A00002295" target="_blank" >RIV/00216208:11320/06:00002295 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The Na+,K+/H+ antiporter Nha1 influences plasma membrane potential of Saccharomyces cerevisiae

Popis výsledku v původním jazyce

There are three different sodium transport systems (Ena1-4p, Nha1p, Nhx1p) in Saccharomyces cerevisiae. The effect of their absence on cell tolerance to alkali-metal cations and on membrane potential was studied. All three sodium transporters were foundto participate in the maintenance of cell Na+, Li+, K+ and Cs+ homeostasis. Measurements of the distribution of a fluorescence potentiometric probe (diS-C3(3) assay) in cell suspensions showed that the lack of all three transporters depolarises the plasma membrane. The overexpression of the Na+,K+/H+ antiporter Nha1 resulted in the hyperpolarisation of the plasma membrane and consequently increased cell sensitivity to Cs+, Tl+ and hygromycin B. This is the first evidence that the activity of a Na+,K+/H+antiporter could play a role in the homeostatic regulation of plasma membrane potential in yeast cells.

Název v anglickém jazyce

The Na+,K+/H+ antiporter Nha1 influences plasma membrane potential of Saccharomyces cerevisiae

Popis výsledku anglicky

There are three different sodium transport systems (Ena1-4p, Nha1p, Nhx1p) in Saccharomyces cerevisiae. The effect of their absence on cell tolerance to alkali-metal cations and on membrane potential was studied. All three sodium transporters were foundto participate in the maintenance of cell Na+, Li+, K+ and Cs+ homeostasis. Measurements of the distribution of a fluorescence potentiometric probe (diS-C3(3) assay) in cell suspensions showed that the lack of all three transporters depolarises the plasma membrane. The overexpression of the Na+,K+/H+ antiporter Nha1 resulted in the hyperpolarisation of the plasma membrane and consequently increased cell sensitivity to Cs+, Tl+ and hygromycin B. This is the first evidence that the activity of a Na+,K+/H+antiporter could play a role in the homeostatic regulation of plasma membrane potential in yeast cells.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEMS Yeast Research

ISSN

1567-1356

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

792-800

Kód UT WoS článku

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EID výsledku v databázi Scopus

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