Magnesium effect on premelting transitions in nucleic acids: DNA duplex and RNA hairpin models

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F11%3A10108119" target="_blank" >RIV/00216208:11320/11:10108119 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0022286010009786" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0022286010009786</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molstruc.2010.12.025" target="_blank" >10.1016/j.molstruc.2010.12.025</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Magnesium effect on premelting transitions in nucleic acids: DNA duplex and RNA hairpin models

Popis výsledku v původním jazyce

The effect of magnesium ions on the conformational changes in the temperature region below the melting transition (premelting transitions) of a DNA duplex and an RNA hairpin was studied by difference Raman spectroscopy. The chosen model systems were thepolynucleotide poly(dA)-poly(dT) duplex and the apical hairpin of the trans-activation response (TAR) element of HIV-1. Magnesium effect was displayed by differences of Raman spectra measured at various temperatures with and without magnesium ions. Our results have revealed that magnesium ions influence measurably the premelting transitions in both cases; the extent of the effect and its character is though quite different. In the case of the B'- B premelting transition of the polynucleotide poly(dA)-poly(dT) duplex, magnesium binds to the minor groove of the B but not of the B0 form and acts again the rearrangement in the vicinity of the thymidine keto-groups. On the other hand, the magnesium effect on the TAR hairpin premelting consis

Název v anglickém jazyce

Magnesium effect on premelting transitions in nucleic acids: DNA duplex and RNA hairpin models

Popis výsledku anglicky

The effect of magnesium ions on the conformational changes in the temperature region below the melting transition (premelting transitions) of a DNA duplex and an RNA hairpin was studied by difference Raman spectroscopy. The chosen model systems were thepolynucleotide poly(dA)-poly(dT) duplex and the apical hairpin of the trans-activation response (TAR) element of HIV-1. Magnesium effect was displayed by differences of Raman spectra measured at various temperatures with and without magnesium ions. Our results have revealed that magnesium ions influence measurably the premelting transitions in both cases; the extent of the effect and its character is though quite different. In the case of the B'- B premelting transition of the polynucleotide poly(dA)-poly(dT) duplex, magnesium binds to the minor groove of the B but not of the B0 form and acts again the rearrangement in the vicinity of the thymidine keto-groups. On the other hand, the magnesium effect on the TAR hairpin premelting consis

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Molecular Structure

ISSN

0022-2860

e-ISSN

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Svazek periodika

993

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

324-327

Kód UT WoS článku

000291066000052

EID výsledku v databázi Scopus

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