Monitoring of real changes of plasma membrane potential by diS-C-3(3) fluorescence in yeast cell suspensions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F12%3A10131297" target="_blank" >RIV/00216208:11320/12:10131297 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10863-012-9458-8" target="_blank" >http://dx.doi.org/10.1007/s10863-012-9458-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10863-012-9458-8" target="_blank" >10.1007/s10863-012-9458-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring of real changes of plasma membrane potential by diS-C-3(3) fluorescence in yeast cell suspensions

Popis výsledku v původním jazyce

The fluorescent dye 3,3'-dipropylthiadicarbocyanine, diS-C-3(3), is a suitable probe to monitor real changes of plasma membrane potential in yeast cells which are too small for direct membrane potential measurements with microelectrodes. A method presented in this paper makes it possible to convert changes of equilibrium diS-C-3(3) fluorescence spectra, measured in yeast cell suspensions under certain defined conditions, into underlying membrane potential differences, scaled in the units of millivolts.Spectral analysis of synchronously scanned diS-C-3(3) fluorescence allows to assess the amount of dye accumulated in cells without otherwise necessary sample taking and following separation of cells from the medium. Moreover, membrane potential changes can be quantified without demanding calibration protocols. The applicability of this approach was demonstrated on the depolarization of Rhodotorula glutinis yeast cells upon acidification of cell suspensions and/or by increasing extracellu

Název v anglickém jazyce

Monitoring of real changes of plasma membrane potential by diS-C-3(3) fluorescence in yeast cell suspensions

Popis výsledku anglicky

The fluorescent dye 3,3'-dipropylthiadicarbocyanine, diS-C-3(3), is a suitable probe to monitor real changes of plasma membrane potential in yeast cells which are too small for direct membrane potential measurements with microelectrodes. A method presented in this paper makes it possible to convert changes of equilibrium diS-C-3(3) fluorescence spectra, measured in yeast cell suspensions under certain defined conditions, into underlying membrane potential differences, scaled in the units of millivolts.Spectral analysis of synchronously scanned diS-C-3(3) fluorescence allows to assess the amount of dye accumulated in cells without otherwise necessary sample taking and following separation of cells from the medium. Moreover, membrane potential changes can be quantified without demanding calibration protocols. The applicability of this approach was demonstrated on the depolarization of Rhodotorula glutinis yeast cells upon acidification of cell suspensions and/or by increasing extracellu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/GAP205%2F10%2F1121" target="_blank" >GAP205/10/1121: Biofyzikální metoda sledování změn membránového potenciálu a aktivity systémů mnohačetné lékové rezistence kvasinek</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Bioenergetics and Biomembranes

ISSN

0145-479X

e-ISSN

—

Svazek periodika

44

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

559-569

Kód UT WoS článku

000308965000004

EID výsledku v databázi Scopus

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