Complementary Methods of Processing diS-C3(3) Fluorescence Spectra Used for Monitoring the Plasma Membrane Potential of Yeast: Their Pros and Cons

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F14%3A10288249" target="_blank" >RIV/00216208:11320/14:10288249 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10895-013-1323-6" target="_blank" >http://dx.doi.org/10.1007/s10895-013-1323-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10895-013-1323-6" target="_blank" >10.1007/s10895-013-1323-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Complementary Methods of Processing diS-C3(3) Fluorescence Spectra Used for Monitoring the Plasma Membrane Potential of Yeast: Their Pros and Cons

Popis výsledku v původním jazyce

Carbocyanine dye diS-C-3(3) was repeatedly employed in monitoring the plasma membrane potential of yeast and other living cells. Four methods of measuring and evaluating probe fluorescence signal were used in different studies, based on following fluorescence parameters: fluorescence intensity emitted within a certain spectral interval, F(580)/F(560) fluorescence emission ratio, wavelength of emissionspectrum maximum, and the ratio of respective fluorescence intensities corresponding to the diS-C-3(3) bound to cytosolic macromolecules and remaining dissolved in the aqueous cell medium (i.e., unbound, or free). We have shown that data corresponding to the three latter spectral assessments of diS-C-3(3) accumulation in cells is mutually convertible, which means that their alternative use cannot lead to ambiguities in the interpretation of the results of biological experiments.

Název v anglickém jazyce

Complementary Methods of Processing diS-C3(3) Fluorescence Spectra Used for Monitoring the Plasma Membrane Potential of Yeast: Their Pros and Cons

Popis výsledku anglicky

Carbocyanine dye diS-C-3(3) was repeatedly employed in monitoring the plasma membrane potential of yeast and other living cells. Four methods of measuring and evaluating probe fluorescence signal were used in different studies, based on following fluorescence parameters: fluorescence intensity emitted within a certain spectral interval, F(580)/F(560) fluorescence emission ratio, wavelength of emissionspectrum maximum, and the ratio of respective fluorescence intensities corresponding to the diS-C-3(3) bound to cytosolic macromolecules and remaining dissolved in the aqueous cell medium (i.e., unbound, or free). We have shown that data corresponding to the three latter spectral assessments of diS-C-3(3) accumulation in cells is mutually convertible, which means that their alternative use cannot lead to ambiguities in the interpretation of the results of biological experiments.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Fluorescence

ISSN

1053-0509

e-ISSN

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Svazek periodika

24

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

541-547

Kód UT WoS článku

000334183100030

EID výsledku v databázi Scopus

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