Spectral- and time-resolved phosphorescence of photosensitizers and singlet oxygen: From in vitro towards in vivo

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F13%3A10173450" target="_blank" >RIV/00216208:11320/13:10173450 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jlumin.2011.12.033" target="_blank" >http://dx.doi.org/10.1016/j.jlumin.2011.12.033</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jlumin.2011.12.033" target="_blank" >10.1016/j.jlumin.2011.12.033</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Spectral- and time-resolved phosphorescence of photosensitizers and singlet oxygen: From in vitro towards in vivo

Popis výsledku v původním jazyce

Spectral- and time-resolved infrared phosphorescence set-up was adapted for detection from surfaces of solid samples by utilizing fiber optics. Its abilities are demonstrated on the detection of singlet oxygen photosensitization by intrinsic (protoporphyrin IX synthesized from ALA) and extrinsic (TPPS4 and TMPyP) photosensitizers in in vitro layers of cultured 3T3 murine fibroblasts and HeLa cells mimicking in vivo tissues. Complex decays of phosphorescence of the photosensitizers were detected. The data were approximated by multi-exponential decays, however, no straightforward explanation of the individual components was found. Singlet oxygen phosphorescence kinetics were obtained with rise-times corresponding to singlet oxygen lifetimes ranging from0.7 mu s to 1.0 mu s and single-exponential decay times between 5.3 mu s and 6.5 mu s for different photosensitizers and cell lines.

Název v anglickém jazyce

Spectral- and time-resolved phosphorescence of photosensitizers and singlet oxygen: From in vitro towards in vivo

Popis výsledku anglicky

Spectral- and time-resolved infrared phosphorescence set-up was adapted for detection from surfaces of solid samples by utilizing fiber optics. Its abilities are demonstrated on the detection of singlet oxygen photosensitization by intrinsic (protoporphyrin IX synthesized from ALA) and extrinsic (TPPS4 and TMPyP) photosensitizers in in vitro layers of cultured 3T3 murine fibroblasts and HeLa cells mimicking in vivo tissues. Complex decays of phosphorescence of the photosensitizers were detected. The data were approximated by multi-exponential decays, however, no straightforward explanation of the individual components was found. Singlet oxygen phosphorescence kinetics were obtained with rise-times corresponding to singlet oxygen lifetimes ranging from0.7 mu s to 1.0 mu s and single-exponential decay times between 5.3 mu s and 6.5 mu s for different photosensitizers and cell lines.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Luminescence

ISSN

0022-2313

e-ISSN

—

Svazek periodika

143

Číslo periodika v rámci svazku

Nov 2013

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

729-733

Kód UT WoS článku

000323912400119

EID výsledku v databázi Scopus

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