Sphingolipid levels crucially modulate lateral microdomain organization of plasma membrane in living yeast

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F14%3A10283120" target="_blank" >RIV/00216208:11320/14:10283120 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/14:00430431

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.febslet.2013.11.038" target="_blank" >http://dx.doi.org/10.1016/j.febslet.2013.11.038</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.febslet.2013.11.038" target="_blank" >10.1016/j.febslet.2013.11.038</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sphingolipid levels crucially modulate lateral microdomain organization of plasma membrane in living yeast

Popis výsledku v původním jazyce

We report sphingolipid-related reorganization of gel-like microdomains in the plasma membrane of living Saccharomyces cerevisiae using trans-Parinaric acid (t-PnA) and 1,6-diphenyl-1,3,5-hexatriene (DPH). Compared to control, the gel-like domains were significantly reduced in the membrane of a sphingolipid-deficient lcb1-100 mutant. The same reduction resulted from sphingolipid depletion by myriocin. The phenotype could be reverted when a myriocin-induced block in sphingolipid biosynthesis was bypassedby exogenous dihydrosphingosine. Lipid order of less-ordered membrane regions decreased with sphingolipid depletion as well, as documented by DPH fluorescence anisotropy. The data indicate that organization of lateral microdomains is an essential physiological role of these structural lipids. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Sphingolipid levels crucially modulate lateral microdomain organization of plasma membrane in living yeast

Popis výsledku anglicky

We report sphingolipid-related reorganization of gel-like microdomains in the plasma membrane of living Saccharomyces cerevisiae using trans-Parinaric acid (t-PnA) and 1,6-diphenyl-1,3,5-hexatriene (DPH). Compared to control, the gel-like domains were significantly reduced in the membrane of a sphingolipid-deficient lcb1-100 mutant. The same reduction resulted from sphingolipid depletion by myriocin. The phenotype could be reverted when a myriocin-induced block in sphingolipid biosynthesis was bypassedby exogenous dihydrosphingosine. Lipid order of less-ordered membrane regions decreased with sphingolipid depletion as well, as documented by DPH fluorescence anisotropy. The data indicate that organization of lateral microdomains is an essential physiological role of these structural lipids. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/GAP205%2F12%2F0720" target="_blank" >GAP205/12/0720: Role membránového potenciálu v laterální organizaci mikrodomén plazmatické membrány</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEBS Letters

ISSN

0014-5793

e-ISSN

—

Svazek periodika

588

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

443-449

Kód UT WoS článku

000329976800010

EID výsledku v databázi Scopus

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