The transmembrane region is responsible for targeting of adaptor protein LAX into "heavy rafts''

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F12%3A00387633" target="_blank" >RIV/68378050:_____/12:00387633 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1371/journal.pone.0036330" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0036330</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0036330" target="_blank" >10.1371/journal.pone.0036330</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The transmembrane region is responsible for targeting of adaptor protein LAX into "heavy rafts''

Popis výsledku v původním jazyce

The importance of membrane compartmentalization into specific membrane microdomains has been shown in many biological processes such as immunoreceptor signaling, membrane trafficking, pathogen infection, and tumor progression. Microdomains like lipid rafts, caveolae and tetraspanin enriched microdomains are relatively resistant to solubilization by some detergents. Large detergent-resistant membrane fragments (DRMs) resulting from such membrane solubilization can be conveniently isolated by density gradient ultracentrifugation or gel filtration. Recently, we described a novel type of raft-like membrane microdomains producing, upon detergent Brij98 solubilization, ?heavy DRMs and containing a number of functionally relevant proteins. Transmembrane adaptor protein LAX is a typical ?heavy raft protein. The present study was designed to identify the molecular determinants targeting LAX-derived constructs to heavy rafts. We identified the transmembrane domain of LAX as a first motif targeti

Název v anglickém jazyce

The transmembrane region is responsible for targeting of adaptor protein LAX into "heavy rafts''

Popis výsledku anglicky

The importance of membrane compartmentalization into specific membrane microdomains has been shown in many biological processes such as immunoreceptor signaling, membrane trafficking, pathogen infection, and tumor progression. Microdomains like lipid rafts, caveolae and tetraspanin enriched microdomains are relatively resistant to solubilization by some detergents. Large detergent-resistant membrane fragments (DRMs) resulting from such membrane solubilization can be conveniently isolated by density gradient ultracentrifugation or gel filtration. Recently, we described a novel type of raft-like membrane microdomains producing, upon detergent Brij98 solubilization, ?heavy DRMs and containing a number of functionally relevant proteins. Transmembrane adaptor protein LAX is a typical ?heavy raft protein. The present study was designed to identify the molecular determinants targeting LAX-derived constructs to heavy rafts. We identified the transmembrane domain of LAX as a first motif targeti

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

000305342300011

EID výsledku v databázi Scopus

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