The singlet-oxygen-sensitized delayed fluorescence in mammalian cells: a time-resolved microscopy approach

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F15%3A10314724" target="_blank" >RIV/00216208:11320/15:10314724 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1039/c4pp00339j" target="_blank" >http://dx.doi.org/10.1039/c4pp00339j</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c4pp00339j" target="_blank" >10.1039/c4pp00339j</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The singlet-oxygen-sensitized delayed fluorescence in mammalian cells: a time-resolved microscopy approach

Popis výsledku v původním jazyce

The present work provides a proof-of-concept that the singlet oxygen-sensitized delayed fluorescence (SOSDF) can be detected from individual living mammalian cells in a time-resolved microscopy experiment. To this end, 3T3 mouse fibroblasts incubated with 100 mu M TPPS4 or TMPyP were used and the microsecond kinetics of the delayed fluorescence (DF) were recorded. The analysis revealed that SOSDF is the major component of the overall DF signal. The microscopy approach enables precise control of experimental conditions - the DF kinetics are clearly influenced by the presence of the O-1(2) quencher (sodium azide), H2O/D2O exchange, and the oxygen concentration. Analysis of SOSDF kinetics, which was reconstructed as a difference DF kinetics between the unquenched and the NaN3-quenched samples, provides a cellular O-1(2) lifetime of tau(Delta) = 1-2 mu s and a TPPS4 triplet lifetime of tau(T) = 22 +/- 5 mu s in agreement with previously published values. The short SOSDF acquisition times,

Název v anglickém jazyce

The singlet-oxygen-sensitized delayed fluorescence in mammalian cells: a time-resolved microscopy approach

Popis výsledku anglicky

The present work provides a proof-of-concept that the singlet oxygen-sensitized delayed fluorescence (SOSDF) can be detected from individual living mammalian cells in a time-resolved microscopy experiment. To this end, 3T3 mouse fibroblasts incubated with 100 mu M TPPS4 or TMPyP were used and the microsecond kinetics of the delayed fluorescence (DF) were recorded. The analysis revealed that SOSDF is the major component of the overall DF signal. The microscopy approach enables precise control of experimental conditions - the DF kinetics are clearly influenced by the presence of the O-1(2) quencher (sodium azide), H2O/D2O exchange, and the oxygen concentration. Analysis of SOSDF kinetics, which was reconstructed as a difference DF kinetics between the unquenched and the NaN3-quenched samples, provides a cellular O-1(2) lifetime of tau(Delta) = 1-2 mu s and a TPPS4 triplet lifetime of tau(T) = 22 +/- 5 mu s in agreement with previously published values. The short SOSDF acquisition times,

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

<a href="/cs/project/GBP501%2F12%2FG055" target="_blank" >GBP501/12/G055: Centrum fotosyntetického výzkumu</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Photochemical and Photobiological Sciences

ISSN

1474-905X

e-ISSN

—

Svazek periodika

14

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

700-713

Kód UT WoS článku

000352283100007

EID výsledku v databázi Scopus

2-s2.0-84926469106