Effect of ribose versus 2 '-deoxyribose residue in guanosine 5 '-monophosphates on formation of G-quartets stabilized by potassium and sodium cations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F16%3A10332426" target="_blank" >RIV/00216208:11320/16:10332426 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.vibspec.2015.12.002" target="_blank" >http://dx.doi.org/10.1016/j.vibspec.2015.12.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vibspec.2015.12.002" target="_blank" >10.1016/j.vibspec.2015.12.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of ribose versus 2 '-deoxyribose residue in guanosine 5 '-monophosphates on formation of G-quartets stabilized by potassium and sodium cations

Popis výsledku v původním jazyce

Raman spectroscopy was used to directly compare different abilities of monomeric ribo- and 2'- deoxyribo guanosine 5'-monophosphates to constitute G-quartets and their supramolecular selfassemblies stabilized by Na+ and K+ cations in pH-neutral or slightly basic aqueous solutions. Nucleotide concentration, stoichiometry and temperature was systematically varied to show that in the presence of Na+ cations, 5'-dGMP remains unordered under the conditions when 5'-rGMP already forms Na+-stabilized G-quartets and soluble ordered assemblies. On the contrary, in the presence of K+ ions both 5'-GMPs readily form self-stacking G-quartets, although self-association of 5'-rGMP is still greater than that of 5'-dGMP. These results are supported by 1H and 31P NMR spectroscopy. The cation-sensitive effect of ribose versus 2'-deoxyribose substitution on the formation of G-quartets and soluble aggregates from monomeric 5'-guanosine monophosphates under physiological pH could be essential for full understanding of different structural polymorphism of RNA and DNA quadruplexes.

Název v anglickém jazyce

Effect of ribose versus 2 '-deoxyribose residue in guanosine 5 '-monophosphates on formation of G-quartets stabilized by potassium and sodium cations

Popis výsledku anglicky

Raman spectroscopy was used to directly compare different abilities of monomeric ribo- and 2'- deoxyribo guanosine 5'-monophosphates to constitute G-quartets and their supramolecular selfassemblies stabilized by Na+ and K+ cations in pH-neutral or slightly basic aqueous solutions. Nucleotide concentration, stoichiometry and temperature was systematically varied to show that in the presence of Na+ cations, 5'-dGMP remains unordered under the conditions when 5'-rGMP already forms Na+-stabilized G-quartets and soluble ordered assemblies. On the contrary, in the presence of K+ ions both 5'-GMPs readily form self-stacking G-quartets, although self-association of 5'-rGMP is still greater than that of 5'-dGMP. These results are supported by 1H and 31P NMR spectroscopy. The cation-sensitive effect of ribose versus 2'-deoxyribose substitution on the formation of G-quartets and soluble aggregates from monomeric 5'-guanosine monophosphates under physiological pH could be essential for full understanding of different structural polymorphism of RNA and DNA quadruplexes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

<a href="/cs/project/GA15-06785S" target="_blank" >GA15-06785S: Regulační úseky nukleových kyselin - polymorfismus, dynamika a interakce</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Vibrational Spectroscopy

ISSN

0924-2031

e-ISSN

—

Svazek periodika

82

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

60-65

Kód UT WoS článku

000371649800010

EID výsledku v databázi Scopus

2-s2.0-84951035033