Studium samoskladby guanosinových kvartetů pomocí spektroskopie vibračního a elektronového cirkulárního dichroismu; nový přístup zkoumání supramolekulárních útvarů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F06%3A00016634" target="_blank" >RIV/60461373:22340/06:00016634 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Investigation of Guanosine Quartet Assemblies by Vibrational and Electronic Circular Dichroism Spectroscopy, a Novel Approach for Studying Supramolecular Entities

Popis výsledku v původním jazyce

The self-assembly of guanosine-5 -hydrazide G-1 in D2O, in the presence and absence of sodium cations, has been investigated by chiroptical techniques: electronic (ECD) and the newly introduced vibrational (VCD) circular dichroism spectroscopy. Using a combination of ECD and VCD with other methods such as IR, electron microscopy, and electrospray ionization mass spectrometry (ESI-MS) it was found that G-1 produces long-range chiral aggregates consisting of G-quartets, (G-1)4, subsequently stacked into columns, [(G-1)4]n, induced by binding of metal cations between the (G-1)4 species. This process, accompanied by gelation of the sample, is highly efficient in the presence of an excess of sodium cations, leading to aggregates with strong quartet-quartetinteraction. Thermally induced conformational changes and conformational stability of guanosine-5 -hydrazide assemblies were studied by chiroptical techniques and the melting temperature of the hydrogels formed was obtained. The temperatu

Název v anglickém jazyce

Investigation of Guanosine Quartet Assemblies by Vibrational and Electronic Circular Dichroism Spectroscopy, a Novel Approach for Studying Supramolecular Entities

Popis výsledku anglicky

The self-assembly of guanosine-5 -hydrazide G-1 in D2O, in the presence and absence of sodium cations, has been investigated by chiroptical techniques: electronic (ECD) and the newly introduced vibrational (VCD) circular dichroism spectroscopy. Using a combination of ECD and VCD with other methods such as IR, electron microscopy, and electrospray ionization mass spectrometry (ESI-MS) it was found that G-1 produces long-range chiral aggregates consisting of G-quartets, (G-1)4, subsequently stacked into columns, [(G-1)4]n, induced by binding of metal cations between the (G-1)4 species. This process, accompanied by gelation of the sample, is highly efficient in the presence of an excess of sodium cations, leading to aggregates with strong quartet-quartetinteraction. Thermally induced conformational changes and conformational stability of guanosine-5 -hydrazide assemblies were studied by chiroptical techniques and the melting temperature of the hydrogels formed was obtained. The temperatu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemistry A European Journal

ISSN

0947-6539

e-ISSN

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Svazek periodika

12

Číslo periodika v rámci svazku

34

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

8735-8743

Kód UT WoS článku

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EID výsledku v databázi Scopus

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