Raman microscopy shows that nitrogen-rich cellular inclusions in microalgae are microcrystalline guanine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F17%3A10368584" target="_blank" >RIV/00216208:11320/17:10368584 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.algal.2017.02.009" target="_blank" >http://dx.doi.org/10.1016/j.algal.2017.02.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.algal.2017.02.009" target="_blank" >10.1016/j.algal.2017.02.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Raman microscopy shows that nitrogen-rich cellular inclusions in microalgae are microcrystalline guanine

Popis výsledku v původním jazyce

Microalgal cells possess a vast diversity of subcellular structures and cytoplasmic inclusions differing in their morphology, functionality, and composition, some of them giving rise to distinct Raman spectral signatures allowing their identification, localization, and visualization in situ. Here, we show that certain Raman features observed in Raman spectra of microalgae can be unambiguously attributed to guanine microcrystals because they are clearly distinct from Raman fingerprints of closely related purine species. Using confocal Raman microscopy, we have localized crystalline guanine as a part of cellular inclusions in the chlorophyte Desmodesmus quadricauda and in the eustigmatophyte Trachydiscus minutus. We propose that this finding also explains the chemical nature of similar nitrogen-rich crystalline structures recently documented in a number of other chlorophyte species by energy-dispersive X-ray spectroscopy. To the best of our knowledge, this is the first Raman microscopy- based direct evidence of the presence of guanine microcrystalline inclusions within microalgal cells. We tentatively propose that the crystalline guanine serves as a very compact, long-termdepot of nitrogen in microalgae. Simplicity of specimen preparation requiring no fixation, labeling, or staining of the cells predetermines Raman microscopy as a method of choice for more advanced studies of the physiological role of guanine particles, as well as other crystalline inclusions in situ within intact cells.

Název v anglickém jazyce

Raman microscopy shows that nitrogen-rich cellular inclusions in microalgae are microcrystalline guanine

Popis výsledku anglicky

Microalgal cells possess a vast diversity of subcellular structures and cytoplasmic inclusions differing in their morphology, functionality, and composition, some of them giving rise to distinct Raman spectral signatures allowing their identification, localization, and visualization in situ. Here, we show that certain Raman features observed in Raman spectra of microalgae can be unambiguously attributed to guanine microcrystals because they are clearly distinct from Raman fingerprints of closely related purine species. Using confocal Raman microscopy, we have localized crystalline guanine as a part of cellular inclusions in the chlorophyte Desmodesmus quadricauda and in the eustigmatophyte Trachydiscus minutus. We propose that this finding also explains the chemical nature of similar nitrogen-rich crystalline structures recently documented in a number of other chlorophyte species by energy-dispersive X-ray spectroscopy. To the best of our knowledge, this is the first Raman microscopy- based direct evidence of the presence of guanine microcrystalline inclusions within microalgal cells. We tentatively propose that the crystalline guanine serves as a very compact, long-termdepot of nitrogen in microalgae. Simplicity of specimen preparation requiring no fixation, labeling, or staining of the cells predetermines Raman microscopy as a method of choice for more advanced studies of the physiological role of guanine particles, as well as other crystalline inclusions in situ within intact cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10301 - Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algal Research

ISSN

2211-9264

e-ISSN

—

Svazek periodika

23

Číslo periodika v rámci svazku

23

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

216-222

Kód UT WoS článku

000397462700024

EID výsledku v databázi Scopus

2-s2.0-85014330086