The Turnover and Possible Physiological Significance of Purine Crystals in the Cells of the Chlorophytes from the Genus Coelastrella (Scenedesmaceae, Chlorophyta)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F24%3A10485693" target="_blank" >RIV/00216208:11320/24:10485693 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=UD678QNe7U" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=UD678QNe7U</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1134/S1021443724606098" target="_blank" >10.1134/S1021443724606098</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Turnover and Possible Physiological Significance of Purine Crystals in the Cells of the Chlorophytes from the Genus Coelastrella (Scenedesmaceae, Chlorophyta)

Popis výsledku v původním jazyce

The productivity of microalgal cultures and their resilience to unfavorable conditions is largely determined by the availability of mineral nutrients, particularly nitrogen. Nitrogen starvation is a strong stressor that induces a broad range of responses in microalgae at the cell and cell population (culture) levels. These responses such as lipogenesis and secondary carotenogenesis are widely used in biotechnology to obtain valuable secondary metabolites of microalgae. It was believed that microalgal cells lack specific structures that function as long-term nitrogen depot, but recent studies assigned this role to microcrystalline inclusions constituted by nitrogenous bases (most often guanine). It is also known that purine microcrystals are biophotonic structures widespread in nature. These structures modify the intensity and spectral composition of radiation in illuminated cells and tissues of living organisms. In this regard, we studied (i) the dynamics of the formation of guanine crystals in the cells of green carotenogenic microalgae from the genus Coelastrella (C. thermophila NAMSU CM1/23 and C. rubescens IPPAS C-2066) depending on the availability of nitrogen in the medium and (ii) the effect of the presence of these crystals on cell resilience to high light intensity. Nitrogen-rich crystal inclusions were accumulated in cells when the cultures reached the stationary growth phase in a medium with ample nitrate nitrogen. During rapid growth, these inclusions were hardly detectable in the cells and disappeared during cultivation in the absence of nitrogen. Optical polarization microscopy and Raman microspectroscopy demonstrated that these nitrogen-rich inclusions are birefringent microcrystals composed of guanine. C. thermophila cells harboring abundant guanine crystals showed resistance to short-term (10-15 min) exposure to high-intensity light (600 mu mol PAR quanta/m(2)/s). The results obtained are discussed in the context of the multifaceted role of guanine crystals in the stress tolerance of microalgae.

Název v anglickém jazyce

The Turnover and Possible Physiological Significance of Purine Crystals in the Cells of the Chlorophytes from the Genus Coelastrella (Scenedesmaceae, Chlorophyta)

Popis výsledku anglicky

The productivity of microalgal cultures and their resilience to unfavorable conditions is largely determined by the availability of mineral nutrients, particularly nitrogen. Nitrogen starvation is a strong stressor that induces a broad range of responses in microalgae at the cell and cell population (culture) levels. These responses such as lipogenesis and secondary carotenogenesis are widely used in biotechnology to obtain valuable secondary metabolites of microalgae. It was believed that microalgal cells lack specific structures that function as long-term nitrogen depot, but recent studies assigned this role to microcrystalline inclusions constituted by nitrogenous bases (most often guanine). It is also known that purine microcrystals are biophotonic structures widespread in nature. These structures modify the intensity and spectral composition of radiation in illuminated cells and tissues of living organisms. In this regard, we studied (i) the dynamics of the formation of guanine crystals in the cells of green carotenogenic microalgae from the genus Coelastrella (C. thermophila NAMSU CM1/23 and C. rubescens IPPAS C-2066) depending on the availability of nitrogen in the medium and (ii) the effect of the presence of these crystals on cell resilience to high light intensity. Nitrogen-rich crystal inclusions were accumulated in cells when the cultures reached the stationary growth phase in a medium with ample nitrate nitrogen. During rapid growth, these inclusions were hardly detectable in the cells and disappeared during cultivation in the absence of nitrogen. Optical polarization microscopy and Raman microspectroscopy demonstrated that these nitrogen-rich inclusions are birefringent microcrystals composed of guanine. C. thermophila cells harboring abundant guanine crystals showed resistance to short-term (10-15 min) exposure to high-intensity light (600 mu mol PAR quanta/m(2)/s). The results obtained are discussed in the context of the multifaceted role of guanine crystals in the stress tolerance of microalgae.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10301 - Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)

Projekt

<a href="/cs/project/GA21-26115S" target="_blank" >GA21-26115S: Guaninové krystaly jako úložiště dusíku v Chromera velia</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Russian Journal of Plant Physiology

ISSN

1021-4437

e-ISSN

1608-3407

Svazek periodika

71

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

VG - Britské Panenské ostrovy

Počet stran výsledku

13

Strana od-do

121

Kód UT WoS článku

001302586400007

EID výsledku v databázi Scopus

2-s2.0-85202849015