Migrující Schwannovy buňky při absenci axonů vykazují reakci na laminin, fibronectin, thrombospondin a chondroitin sulfat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F00%3A00002251" target="_blank" >RIV/00216224:14110/00:00002251 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Schwann cells migrating in absence of the axons in the basal lamina tubes express immunoreactivity for laminins, fibronectin, thrombospondin, and chondroitin sulfate

Popis výsledku v původním jazyce

The acellular nerve segment is thought to be a method of choice for reparation of nerve defect. Repeated periods of freezing and thawing are frequently used for preparation of standard acellular nerve segment in experimental application. It was believedthat the basal lamina tubes and endoneurial extracellular matrix molecules survive the procedures and promote the growth of regenerating axons. We used the model of freeze-treated segment of the distal nerve stump to study a molecular content of the endoneurium in absence and presence of Schwann cells. The nerve segment, marked out at the end of distal stump of transected rat sciatic nerve, was repeatedly frozen and thawed (5 times) to evacuate all resident cells. Serial longitudinal frozen sections, 10microm thick, were incubated with monoclonal antibodies against laminin-1, -2, and -3, fibronectin, thrombospondin, as well as chondroitin sulfate. The position of Schwann cells was detected in subsequent sections or simultaneously by im

Název v anglickém jazyce

Schwann cells migrating in absence of the axons in the basal lamina tubes express immunoreactivity for laminins, fibronectin, thrombospondin, and chondroitin sulfate

Popis výsledku anglicky

The acellular nerve segment is thought to be a method of choice for reparation of nerve defect. Repeated periods of freezing and thawing are frequently used for preparation of standard acellular nerve segment in experimental application. It was believedthat the basal lamina tubes and endoneurial extracellular matrix molecules survive the procedures and promote the growth of regenerating axons. We used the model of freeze-treated segment of the distal nerve stump to study a molecular content of the endoneurium in absence and presence of Schwann cells. The nerve segment, marked out at the end of distal stump of transected rat sciatic nerve, was repeatedly frozen and thawed (5 times) to evacuate all resident cells. Serial longitudinal frozen sections, 10microm thick, were incubated with monoclonal antibodies against laminin-1, -2, and -3, fibronectin, thrombospondin, as well as chondroitin sulfate. The position of Schwann cells was detected in subsequent sections or simultaneously by im

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

—

Projekt

<a href="/cs/project/GA309%2F00%2F0407" target="_blank" >GA309/00/0407: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Eur. J. Neurosci.

ISSN

0953-816X

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

Suppl. 11

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

1

Strana od-do

—

Kód UT WoS článku

000088236601655

EID výsledku v databázi Scopus

—