Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F00%3A00002596" target="_blank" >RIV/00216224:14110/00:00002596 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate.

Popis výsledku v původním jazyce

The acellular nerve segment is thought to be a method of choice for reparation of nerve defect. Repeated periods of freezing and thawing are frequently used for preparation of the standard acellular nerve segment in experimental application. It was believed that the basal lamina tubes and endoneurial extracellular matrix molecules survive the procedures and promote the growth of regenerating axons. We used a model of the freeze-treated nerve segment to study the molecular content of the endoneurium in absence and presence of Schwann cells. The sciatic nerves of 10 adult female rats were cut and the 10 mm long nerve segments were frozen 5 times with forceps pre-cooled in liquid nitrogen. The rats were secrificed after 14 days. Serial longitudinal frozensections, 10 ľm thick, were incubated with antibodies against S-100 protein, laminin-1, -2, and -3, fibronectin, thrombospondin, as well as chondroitin sulfate. The binding of primary antibodies was detected with indirect biotin-streptavi

Název v anglickém jazyce

Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate.

Popis výsledku anglicky

The acellular nerve segment is thought to be a method of choice for reparation of nerve defect. Repeated periods of freezing and thawing are frequently used for preparation of the standard acellular nerve segment in experimental application. It was believed that the basal lamina tubes and endoneurial extracellular matrix molecules survive the procedures and promote the growth of regenerating axons. We used a model of the freeze-treated nerve segment to study the molecular content of the endoneurium in absence and presence of Schwann cells. The sciatic nerves of 10 adult female rats were cut and the 10 mm long nerve segments were frozen 5 times with forceps pre-cooled in liquid nitrogen. The rats were secrificed after 14 days. Serial longitudinal frozensections, 10 ľm thick, were incubated with antibodies against S-100 protein, laminin-1, -2, and -3, fibronectin, thrombospondin, as well as chondroitin sulfate. The binding of primary antibodies was detected with indirect biotin-streptavi

Druh

D - Stať ve sborníku

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Sborník lékařský

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

Karlova Univerzita

Místo vydání

Praha

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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