Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F01%3A00004307" target="_blank" >RIV/00216224:14110/01:00004307 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/01:00004972

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells

Popis výsledku v původním jazyce

We compared the effects of four quaternary benzo[c]phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine, and two quaternary protoberberine alkaloids, berberine and coptisine, on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomyces japonicus var. versatilis. The ability of alkaloids to display primary fluorescence, allowed us to record their dynamics and localization in cells. Cytotoxicity, anti-microtubularand anti-actin effects in living cells were studied. In the yeasts, neither microtubules nor cell growth were seriously affected even at the alkaloid concentration of 100 mg/mL. The HeLa cells, however, responded to the toxic effect of alkaloids at concentrations ranging from 1-50 mg/mL. IC50 values for individual alkaloids were: sanguinarine IC50 = 0.8 mg/mL, sanguilutine IC50 = 8.3 mg/mL, chelerythrine IC50 = 6.2 mg/mL, chelilutine IC50 = 5.2 mg/mL, coptisine IC50 = 2.6 mg/mL and berb

Název v anglickém jazyce

Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells

Popis výsledku anglicky

We compared the effects of four quaternary benzo[c]phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine, and two quaternary protoberberine alkaloids, berberine and coptisine, on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomyces japonicus var. versatilis. The ability of alkaloids to display primary fluorescence, allowed us to record their dynamics and localization in cells. Cytotoxicity, anti-microtubularand anti-actin effects in living cells were studied. In the yeasts, neither microtubules nor cell growth were seriously affected even at the alkaloid concentration of 100 mg/mL. The HeLa cells, however, responded to the toxic effect of alkaloids at concentrations ranging from 1-50 mg/mL. IC50 values for individual alkaloids were: sanguinarine IC50 = 0.8 mg/mL, sanguilutine IC50 = 8.3 mg/mL, chelerythrine IC50 = 6.2 mg/mL, chelilutine IC50 = 5.2 mg/mL, coptisine IC50 = 2.6 mg/mL and berb

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA204%2F99%2FD025" target="_blank" >GA204/99/D025: Úloha cytoskeletu a buněčné stěny v polarizaci buňky</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell Biology and Toxicology

ISSN

0742-2091

e-ISSN

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Svazek periodika

17

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

51

Kód UT WoS článku

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EID výsledku v databázi Scopus

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