Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F10%3A00043675" target="_blank" >RIV/00216224:14110/10:00043675 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells

Popis výsledku v původním jazyce

Sanguilutine and chelilutine, quaternary benzo[c]phenanthridine alkaloids, were studied for their anti-proliferative activities with regard to their ability to induce oxidative stress. We observed potent anti-proliferative activities for both alkaloids against three tumor (HeLa; HL-60; A-2780) and two normal (V-79; LEP) cell lines. Both alkaloids were efficient inductors of apoptosis. Statistical analysis revealed higher toxicity for sanguilutine compared to chelilutine and an unequal sensitivity with regard to individual cell lines but independent of the character of the cell line (i.e. tumor vs. normal). Dihydrofluorescein diacetate staining was used for measurement of intracellular ROS accumulation, not only after sanguilutine and chelilutine, but also after sanguinarine and chelerythrine treatment. In addition the antioxidative effect was studied. The effect of the alkaloids was compared with the effect of commonly used antioxidants such as trolox, caffeine acid, and chlorogenic ac

Název v anglickém jazyce

Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells

Popis výsledku anglicky

Sanguilutine and chelilutine, quaternary benzo[c]phenanthridine alkaloids, were studied for their anti-proliferative activities with regard to their ability to induce oxidative stress. We observed potent anti-proliferative activities for both alkaloids against three tumor (HeLa; HL-60; A-2780) and two normal (V-79; LEP) cell lines. Both alkaloids were efficient inductors of apoptosis. Statistical analysis revealed higher toxicity for sanguilutine compared to chelilutine and an unequal sensitivity with regard to individual cell lines but independent of the character of the cell line (i.e. tumor vs. normal). Dihydrofluorescein diacetate staining was used for measurement of intracellular ROS accumulation, not only after sanguilutine and chelilutine, but also after sanguinarine and chelerythrine treatment. In addition the antioxidative effect was studied. The effect of the alkaloids was compared with the effect of commonly used antioxidants such as trolox, caffeine acid, and chlorogenic ac

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology in Vitro

ISSN

0887-2333

e-ISSN

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Svazek periodika

24

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

000276668500001

EID výsledku v databázi Scopus

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