Intra- a Extraneuronální změny v imunofluorescenci TNF-a a TNFR1 ve spinálních gangliích potkana v modelech periferní neuropatické bolesti

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F06%3A00015796" target="_blank" >RIV/00216224:14110/06:00015796 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models

Popis výsledku v původním jazyce

1. Several lines of evidence suggest that cytokines and their receptors are initiators of changes in the activity of dorsal root ganglia (DRG) neurons, but their cellular distribution is still very limited or controversial. Therefore, the goal of presentstudy was to investigate immunohistochemical distribution of TNF-a and TNF receptor-1 (TNFR1) proteins in the rat DRG following three types of nerve injury. 2. The unilateral sciatic and spinal nerve ligation as well as the sciatic nerve transection were used to induce changes in the distribution of TNF-a and TNFR1 proteins. The TNF-a and TNFR1 immunofluorescence was assessed in the L4-L5 DRG affected by nerve injury for 1 and 2 weeks, and compared with the contralateral ones and those removed from naive or sham-operated rats. A part of the sections was incubated for simultaneous immunostaining for TNF-a and ED-1. The immunofluorescence brightness was measured by image analysis system (LUCIA-G v4.21) to quantify immunostaining for TNF-

Název v anglickém jazyce

Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models

Popis výsledku anglicky

1. Several lines of evidence suggest that cytokines and their receptors are initiators of changes in the activity of dorsal root ganglia (DRG) neurons, but their cellular distribution is still very limited or controversial. Therefore, the goal of presentstudy was to investigate immunohistochemical distribution of TNF-a and TNF receptor-1 (TNFR1) proteins in the rat DRG following three types of nerve injury. 2. The unilateral sciatic and spinal nerve ligation as well as the sciatic nerve transection were used to induce changes in the distribution of TNF-a and TNFR1 proteins. The TNF-a and TNFR1 immunofluorescence was assessed in the L4-L5 DRG affected by nerve injury for 1 and 2 weeks, and compared with the contralateral ones and those removed from naive or sham-operated rats. A part of the sections was incubated for simultaneous immunostaining for TNF-a and ED-1. The immunofluorescence brightness was measured by image analysis system (LUCIA-G v4.21) to quantify immunostaining for TNF-

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

<a href="/cs/project/GA309%2F03%2F1199" target="_blank" >GA309/03/1199: Změny v satelitních gliových buňkách a jejich extracelulární matrix po axotomii - podmínky pro tvorbu sympatických kolaterál ve spinálních gangliích ?</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cellular and Molecular Neurobiology

ISSN

1573-6830

e-ISSN

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Svazek periodika

26

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

365-378

Kód UT WoS článku

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EID výsledku v databázi Scopus

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