VPLYV METANANDAMIDU NA AKTIVITU PEČEŇOVÝCH IZOENZÝMOV CYP2D2 A 1A2 V PREKLINICKOM EXPERIMENTE

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F07%3A00023397" target="_blank" >RIV/00216224:14110/07:00023397 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

THE INFLUENCE OF CANNABINOID METHANANDAMIDE ON THE ACTIVITY OF HEPATIC CYP2D2 IN RATS

Popis výsledku v původním jazyce

Anandamide, an endogenous ligand for brain cannabinoid CB1 receptor, produces many behavioral effects similar to those of 9-tetrahydrocannabinol (THC), the main psychoactive ingredient in marijuana. (R)-(+)-methanandamide (MET) is a synthetic long-lasting arachidonylethanolamide (anandamide) analog that is metabolically stable and displays higher affinity for the cannabinoid receptor. The aim of this work was to investigate the effect of repeated administration of MET on cytochrome P450 (CYP450) mediated biotransformation in rat. The enzymatic system of CYP450 is a part of phase I of enzymatic biotransformation. CYP450 metabolizes many drugs including the major psychoactive cannabinoid present in marijuana THC. We have studied the influence of (R)-(+)-methanandamide on rat liver CYP2D2 isoenzyme using dextromethorphan as a specific marker. As we expected, the activity of CYP2D2 differed due to pretreatment with methanandamide. In our experiments the influence of this compound on rat CY

Název v anglickém jazyce

THE INFLUENCE OF CANNABINOID METHANANDAMIDE ON THE ACTIVITY OF HEPATIC CYP2D2 IN RATS

Popis výsledku anglicky

Anandamide, an endogenous ligand for brain cannabinoid CB1 receptor, produces many behavioral effects similar to those of 9-tetrahydrocannabinol (THC), the main psychoactive ingredient in marijuana. (R)-(+)-methanandamide (MET) is a synthetic long-lasting arachidonylethanolamide (anandamide) analog that is metabolically stable and displays higher affinity for the cannabinoid receptor. The aim of this work was to investigate the effect of repeated administration of MET on cytochrome P450 (CYP450) mediated biotransformation in rat. The enzymatic system of CYP450 is a part of phase I of enzymatic biotransformation. CYP450 metabolizes many drugs including the major psychoactive cannabinoid present in marijuana THC. We have studied the influence of (R)-(+)-methanandamide on rat liver CYP2D2 isoenzyme using dextromethorphan as a specific marker. As we expected, the activity of CYP2D2 differed due to pretreatment with methanandamide. In our experiments the influence of this compound on rat CY

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical Papers

ISSN

1213-8118

e-ISSN

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Svazek periodika

151

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

2

Strana od-do

103-104

Kód UT WoS článku

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EID výsledku v databázi Scopus

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