Charakterizace lidských embryonálních kmenových buněk mezinárodní iniciativou pro kmenové buňky

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F07%3A00023516" target="_blank" >RIV/00216224:14110/07:00023516 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/07:00091129

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

Popis výsledku v původním jazyce

This study represents the result of the first international comparison of a large number of lines of human embryonic (ES) stem cells from laboratories worldwide (International Stem Cell Initiative) that has been accomplished with the aim to reveal and characterize their similarities and/or differences. In this study, we have analyzed 59 independently derived human ES cell lines, from 17 laboratories in 11 countries, for expression of 17 cell-surface antigens and 93 genes, chosen as potential markers ofthe undifferentiated stem cells or their differentiation derivatives. In adition, the allele-specific expression of status of ten imprinted genes was examined in those cell lines with distinct alleles. We have also assessed the microbiological status ofthe cell lines and reviewed the histology of a sample of xenograft teratomas to in vivo judge the differentiation potential of the cells. Our data show that despite diverse genotypes and different techniques used for derivation and mainte

Název v anglickém jazyce

Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

Popis výsledku anglicky

This study represents the result of the first international comparison of a large number of lines of human embryonic (ES) stem cells from laboratories worldwide (International Stem Cell Initiative) that has been accomplished with the aim to reveal and characterize their similarities and/or differences. In this study, we have analyzed 59 independently derived human ES cell lines, from 17 laboratories in 11 countries, for expression of 17 cell-surface antigens and 93 genes, chosen as potential markers ofthe undifferentiated stem cells or their differentiation derivatives. In adition, the allele-specific expression of status of ten imprinted genes was examined in those cell lines with distinct alleles. We have also assessed the microbiological status ofthe cell lines and reviewed the histology of a sample of xenograft teratomas to in vivo judge the differentiation potential of the cells. Our data show that despite diverse genotypes and different techniques used for derivation and mainte

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature biotechnology

ISSN

1087-0156

e-ISSN

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Svazek periodika

25

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

803-816

Kód UT WoS článku

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EID výsledku v databázi Scopus

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