Knockdown of oncogenic microRNA-21 displays cytotoxicity in p53 wild-type colon cancer cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F08%3A00028238" target="_blank" >RIV/00216224:14110/08:00028238 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Knockdown of oncogenic microRNA-21 displays cytotoxicity in p53 wild-type colon cancer cells

Popis výsledku v původním jazyce

Although the number of verified human microRNAs (miRNAs) is still expanding, only few have been functionally described. However, emerging evidences suggest the involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous data suggest altered regulation of microRNA-21 (miR-21) expression in CRC. In our study, we examined by Real-Time PCR expression levels of microRNA-21 (miR-21) in 60 colorectal tumors and40 paired adjacent non-tumor tissues and correlated them to selected clinicopathologic features and survival parameters. We used expression of U6 small nuclear RNA (RNU6B) for data normalization and standard ddCt method for relative quantification of miRNA expression. Levels of miR-21 were significantly higher in tumors comparing to normal mucosa (p &lt; 0,0001, Wilcoxon matched-pairs test). High expression levels of miR-21 in tumors (based on high tertile) were associated also with a p

Název v anglickém jazyce

Knockdown of oncogenic microRNA-21 displays cytotoxicity in p53 wild-type colon cancer cells

Popis výsledku anglicky

Although the number of verified human microRNAs (miRNAs) is still expanding, only few have been functionally described. However, emerging evidences suggest the involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous data suggest altered regulation of microRNA-21 (miR-21) expression in CRC. In our study, we examined by Real-Time PCR expression levels of microRNA-21 (miR-21) in 60 colorectal tumors and40 paired adjacent non-tumor tissues and correlated them to selected clinicopathologic features and survival parameters. We used expression of U6 small nuclear RNA (RNU6B) for data normalization and standard ddCt method for relative quantification of miRNA expression. Levels of miR-21 were significantly higher in tumors comparing to normal mucosa (p &lt; 0,0001, Wilcoxon matched-pairs test). High expression levels of miR-21 in tumors (based on high tertile) were associated also with a p

Druh

D - Stať ve sborníku

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/NR9076" target="_blank" >NR9076: Genomické profilování v predikci odpovědi na chemoradioterapii u pacientů s lokálně pokročilým karcinomem konečníku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

European Journal of Cancer Supplements

ISBN

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ISSN

1359-6349

e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

Elsevier Ltd.

Místo vydání

Oxford, UK

Místo konání akce

Lyon, France

Datum konání akce

5. 8. 2008

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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