Significance of microRNA-21 in colorectal cancer pathogenesis.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F08%3A00028239" target="_blank" >RIV/00216224:14110/08:00028239 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Significance of microRNA-21 in colorectal cancer pathogenesis.

Popis výsledku v původním jazyce

MicroRNAs (miRNAs) are endogenously expressed short non-coding RNAs, that repress protein translation through binding to target mRNAs. Although the number of verified human miRNA is still expanding, only few have been functionally described. However, emerging evidences suggest the involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous studies, mainly based on microarrays technology applied on colorectal cancer cell lines, showed altered expression levels of several miRNAs in colorectal cancer (CRC). In our study, we examined by Real-Time PCR expression levels of microRNA-21 (miR-21) in tumors of 29 colorectal cancer patients. For 6 cases of CRC samples also adjacent non-tumor tissues were analyzed. For data normalization we tried different approaches (18S rRNA, GAPDH, let-7a-1). Finally, variability of let-7a-1 expression was shown to be the lowest. Expression levels of miR-21 were

Název v anglickém jazyce

Significance of microRNA-21 in colorectal cancer pathogenesis.

Popis výsledku anglicky

MicroRNAs (miRNAs) are endogenously expressed short non-coding RNAs, that repress protein translation through binding to target mRNAs. Although the number of verified human miRNA is still expanding, only few have been functionally described. However, emerging evidences suggest the involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous studies, mainly based on microarrays technology applied on colorectal cancer cell lines, showed altered expression levels of several miRNAs in colorectal cancer (CRC). In our study, we examined by Real-Time PCR expression levels of microRNA-21 (miR-21) in tumors of 29 colorectal cancer patients. For 6 cases of CRC samples also adjacent non-tumor tissues were analyzed. For data normalization we tried different approaches (18S rRNA, GAPDH, let-7a-1). Finally, variability of let-7a-1 expression was shown to be the lowest. Expression levels of miR-21 were

Druh

D - Stať ve sborníku

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/NR9076" target="_blank" >NR9076: Genomické profilování v predikci odpovědi na chemoradioterapii u pacientů s lokálně pokročilým karcinomem konečníku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

FEBS Journal

ISBN

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ISSN

1742-464X

e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

FEBS

Místo vydání

Oxford, UK

Místo konání akce

Athens

Datum konání akce

1. 1. 2008

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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