Differentiation of Staphylococcus spp. by High-Resolution Melting Analysis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F10%3A00051710" target="_blank" >RIV/00216224:14110/10:00051710 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00027162:_____/11:#0000749
Výsledek na webu
<a href="http://dx.doi.org/10.1139/W10-091" target="_blank" >http://dx.doi.org/10.1139/W10-091</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1139/W10-091" target="_blank" >10.1139/W10-091</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Differentiation of Staphylococcus spp. by High-Resolution Melting Analysis
Popis výsledku v původním jazyce
High-resolution melting analysis (HRMA) is a fast high-throughput method to scan for sequence variations in a target gene. The aim of this study was to test the potential of HRMA to distinguish particular bacterial species of the Staphylococcus genus even when using a broad-range PCR within the 16S rRNA gene where sequence differences are minimal. Genomic DNA samples isolated from 12 reference strains were subjected to a real-time PCR amplification of the 16S rRNA gene in the presence of fluorescent dye, followed by HRMA. Melting profiles were used as molecular fingerprints for bacterial species differentiation. HRMA of S. saprophyticus and S. xylosus resulted in undistinguishable profiles because of their identical sequences in the analyzed 16S rRNA region. The remaining reference strains were fully differentiated either directly or via high-resolution plots obtained by heteroduplex formation between coamplified PCR products of the tested staphylococcal strain and phylogenetically unr
Název v anglickém jazyce
Differentiation of Staphylococcus spp. by High-Resolution Melting Analysis
Popis výsledku anglicky
High-resolution melting analysis (HRMA) is a fast high-throughput method to scan for sequence variations in a target gene. The aim of this study was to test the potential of HRMA to distinguish particular bacterial species of the Staphylococcus genus even when using a broad-range PCR within the 16S rRNA gene where sequence differences are minimal. Genomic DNA samples isolated from 12 reference strains were subjected to a real-time PCR amplification of the 16S rRNA gene in the presence of fluorescent dye, followed by HRMA. Melting profiles were used as molecular fingerprints for bacterial species differentiation. HRMA of S. saprophyticus and S. xylosus resulted in undistinguishable profiles because of their identical sequences in the analyzed 16S rRNA region. The remaining reference strains were fully differentiated either directly or via high-resolution plots obtained by heteroduplex formation between coamplified PCR products of the tested staphylococcal strain and phylogenetically unr
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Canadian Journal of Microbiology
ISSN
0008-4166
e-ISSN
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Svazek periodika
56
Číslo periodika v rámci svazku
12
Stát vydavatele periodika
CA - Kanada
Počet stran výsledku
10
Strana od-do
1040-1049
Kód UT WoS článku
000285556000008
EID výsledku v databázi Scopus
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