Ultrastructural disorder of actin mutant suggests uncoupling of actin-dependent pathway from microtubule-dependent pathway in budding yeast

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F11%3A00050081" target="_blank" >RIV/00216224:14110/11:00050081 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1093/jmicro/dfr073" target="_blank" >http://dx.doi.org/10.1093/jmicro/dfr073</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jmicro/dfr073" target="_blank" >10.1093/jmicro/dfr073</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ultrastructural disorder of actin mutant suggests uncoupling of actin-dependent pathway from microtubule-dependent pathway in budding yeast

Popis výsledku v původním jazyce

Temperature-sensitive actin mutant of Saccharomyces cerevisiae act1-1 was studied at a permissive temperature of 23 C by light, fluorescent and electron microscopy to elucidate the roles of actin cytoskeleton in the cycling eukaryotic cells. Mutant cellsthat grew slowly at the permissive temperature showed aberrations in the cytoskeleton and cell cycle. Mutant cells contained aberrant faint actin cables, that failed in directing of mitochondria, vacuoles and secretory vesicles to the bud and the strayvesicles delivered their content to the mother wall instead of the bud. Bud growth was delayed. Spindle pole bodies and cytoplasmic microtubules did not direct to the bud, and nucleus failed to migrate to the bud. Repeated nuclear divisions produced multinucleated cells, indicating continued cycling of actin mutant cells that failed in the morphogenetic checkpoint, the spindle position checkpoint and cytokinesis.

Název v anglickém jazyce

Ultrastructural disorder of actin mutant suggests uncoupling of actin-dependent pathway from microtubule-dependent pathway in budding yeast

Popis výsledku anglicky

Temperature-sensitive actin mutant of Saccharomyces cerevisiae act1-1 was studied at a permissive temperature of 23 C by light, fluorescent and electron microscopy to elucidate the roles of actin cytoskeleton in the cycling eukaryotic cells. Mutant cellsthat grew slowly at the permissive temperature showed aberrations in the cytoskeleton and cell cycle. Mutant cells contained aberrant faint actin cables, that failed in directing of mitochondria, vacuoles and secretory vesicles to the bud and the strayvesicles delivered their content to the mother wall instead of the bud. Bud growth was delayed. Spindle pole bodies and cytoplasmic microtubules did not direct to the bud, and nucleus failed to migrate to the bud. Repeated nuclear divisions produced multinucleated cells, indicating continued cycling of actin mutant cells that failed in the morphogenetic checkpoint, the spindle position checkpoint and cytokinesis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA310%2F06%2F0605" target="_blank" >GA310/06/0605: Lidské patogenní houby : cytoskelet jako potenciální terč inhibice morfogeneze.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Electron Microscopy

ISSN

0022-0744

e-ISSN

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Svazek periodika

60

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

JP - Japonsko

Počet stran výsledku

13

Strana od-do

379-391

Kód UT WoS článku

000297867800005

EID výsledku v databázi Scopus

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