Ultrastructural disorder of the secretory pathway in temperature-sensitive actin mutants of Saccharomyces cerevisiae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F10%3A00049621" target="_blank" >RIV/00216224:14110/10:00049621 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1093/jmicro/dfp050" target="_blank" >http://dx.doi.org/10.1093/jmicro/dfp050</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jmicro/dfp050" target="_blank" >10.1093/jmicro/dfp050</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ultrastructural disorder of the secretory pathway in temperature-sensitive actin mutants of Saccharomyces cerevisiae

Popis výsledku v původním jazyce

Phenotypes of the two temperature-sensitive actin mutants of Saccharomyces cerevisiae act1-1 and act1-2 at permissive, restrictive and semirestrictive temperatures were studied by freeze fracture and thin section electron micr., and fluoresc. micr. In contrast to secretory mutants where accumulations of either secretory vesicles, Golgi app., or end. reticulum were reported, act1-1 and act1-2 mutants revealed accumulation of all the three components, even at 23C. However, more distinct accumulation of secretory organelles was evident during cultivation at the sub-restrictive temperature of 30C. At temperature of 37C, many cells died, and their empty cell walls remained. Some of the few living cells showed features of apoptosis. From the present study, actin cables are concluded to be necessary for (i) correct spatial positioning and orientation of secretary pathway to the bud and septum, and (ii) vectorial movement of vesicles of the secretory pathway along the actin cables to the bud a

Název v anglickém jazyce

Ultrastructural disorder of the secretory pathway in temperature-sensitive actin mutants of Saccharomyces cerevisiae

Popis výsledku anglicky

Phenotypes of the two temperature-sensitive actin mutants of Saccharomyces cerevisiae act1-1 and act1-2 at permissive, restrictive and semirestrictive temperatures were studied by freeze fracture and thin section electron micr., and fluoresc. micr. In contrast to secretory mutants where accumulations of either secretory vesicles, Golgi app., or end. reticulum were reported, act1-1 and act1-2 mutants revealed accumulation of all the three components, even at 23C. However, more distinct accumulation of secretory organelles was evident during cultivation at the sub-restrictive temperature of 30C. At temperature of 37C, many cells died, and their empty cell walls remained. Some of the few living cells showed features of apoptosis. From the present study, actin cables are concluded to be necessary for (i) correct spatial positioning and orientation of secretary pathway to the bud and septum, and (ii) vectorial movement of vesicles of the secretory pathway along the actin cables to the bud a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

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Projekt

<a href="/cs/project/GA310%2F06%2F0605" target="_blank" >GA310/06/0605: Lidské patogenní houby : cytoskelet jako potenciální terč inhibice morfogeneze.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Electron Microscopy

ISSN

0022-0744

e-ISSN

—

Svazek periodika

59

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

JP - Japonsko

Počet stran výsledku

12

Strana od-do

141-152

Kód UT WoS článku

000276527700006

EID výsledku v databázi Scopus

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