Molecular detection and typing of Treponema pallidum ssp. pallidum in clinical samples based on sequencing of TP0136, TP0548 and 23S rRNA genes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F11%3A00050392" target="_blank" >RIV/00216224:14110/11:00050392 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Molecular detection and typing of Treponema pallidum ssp. pallidum in clinical samples based on sequencing of TP0136, TP0548 and 23S rRNA genes
Popis výsledku v původním jazyce
Syphilis, caused by the spirochete Treponema pallidum subsp. pallidum (TPA), is a sexually transmitted infectious disease with worldwide occurrence. The bacterium TPA cannot be cultivated in vitro, therefore laboratory diagnostics is traditionally made by microscopy and serology testing, which do not provide epidemiological data. Molecular detection of treponemes could type the clinical samples and provide the antibiotic susceptibility information. During the years 2004-2010, 294 patients (415 samples)were tested for the presence of treponemal DNA. Primary screening of clinical specimens included nested PCR detection of two TPA specific loci (tmpC and polA genes). Out of 294 patients, 91 patients were PCR positive. PCR positive patients were more often in the primary stage of syphilis (p=0.0003) compared to the control (PCR negative) group of syphilis seropositive patients.
Název v anglickém jazyce
Molecular detection and typing of Treponema pallidum ssp. pallidum in clinical samples based on sequencing of TP0136, TP0548 and 23S rRNA genes
Popis výsledku anglicky
Syphilis, caused by the spirochete Treponema pallidum subsp. pallidum (TPA), is a sexually transmitted infectious disease with worldwide occurrence. The bacterium TPA cannot be cultivated in vitro, therefore laboratory diagnostics is traditionally made by microscopy and serology testing, which do not provide epidemiological data. Molecular detection of treponemes could type the clinical samples and provide the antibiotic susceptibility information. During the years 2004-2010, 294 patients (415 samples)were tested for the presence of treponemal DNA. Primary screening of clinical specimens included nested PCR detection of two TPA specific loci (tmpC and polA genes). Out of 294 patients, 91 patients were PCR positive. PCR positive patients were more often in the primary stage of syphilis (p=0.0003) compared to the control (PCR negative) group of syphilis seropositive patients.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů