Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F12%3A00057856" target="_blank" >RIV/00216224:14110/12:00057856 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/75010330:_____/12:00009890 RIV/00216208:11110/12:12101 RIV/00064165:_____/12:12101
Výsledek na webu
<a href="http://dx.doi.org/10.2340/00015555-1335" target="_blank" >http://dx.doi.org/10.2340/00015555-1335</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2340/00015555-1335" target="_blank" >10.2340/00015555-1335</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain
Popis výsledku v původním jazyce
A set of 415 clinical samples isolated from 294 patients suspected of having syphilis collected in the Czech Republic between 2004 and 2010 was tested for the presence of treponemal DNA. Standard serological tests showed that 197 patients were syphilis-seropositive and 97 patients were syphilis-seronegative. In each sample, PCR tests for polA (TP0105), tmpC (TP0319), TP0136, TP0548 and 23S rRNA genes were performed. Samples taken from 91 patients were PCR-positive. Molecular typing of treponemal DNA wasbased on the sequencing of TP0136, TP0548 and 23S rRNA genes. Treponemal DNA was typeable in samples taken from 64 PCR-positive patients and 9 different genotypes were found. The proportion of treponemal strains resistant to macrolide antibiotics was 37.3%. In the DNA samples taken from 39 patients, a parallel treponemal typing approved by Centers for Disease Control and Prevention was performed.
Název v anglickém jazyce
Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain
Popis výsledku anglicky
A set of 415 clinical samples isolated from 294 patients suspected of having syphilis collected in the Czech Republic between 2004 and 2010 was tested for the presence of treponemal DNA. Standard serological tests showed that 197 patients were syphilis-seropositive and 97 patients were syphilis-seronegative. In each sample, PCR tests for polA (TP0105), tmpC (TP0319), TP0136, TP0548 and 23S rRNA genes were performed. Samples taken from 91 patients were PCR-positive. Molecular typing of treponemal DNA wasbased on the sequencing of TP0136, TP0548 and 23S rRNA genes. Treponemal DNA was typeable in samples taken from 64 PCR-positive patients and 9 different genotypes were found. The proportion of treponemal strains resistant to macrolide antibiotics was 37.3%. In the DNA samples taken from 39 patients, a parallel treponemal typing approved by Centers for Disease Control and Prevention was performed.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Acta Dermato-Venereologica
ISSN
0001-5555
e-ISSN
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Svazek periodika
92
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
6
Strana od-do
669-674
Kód UT WoS článku
000310828300030
EID výsledku v databázi Scopus
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