Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genogypes are Related to the Sequence of the SS14 Strain

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F12%3A%230001785" target="_blank" >RIV/65269705:_____/12:#0001785 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2340/0001555-1335" target="_blank" >http://dx.doi.org/10.2340/0001555-1335</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2340/00015555-1335" target="_blank" >10.2340/00015555-1335</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genogypes are Related to the Sequence of the SS14 Strain

Popis výsledku v původním jazyce

A set of 415 clinical samples isolated from 294 patients suspected of having syphilis collected in the Czech Republic between 2004 and 2010 was tested for the presence of treponemal DNA. Standard serological tests showed that 197 patients were syphilis-seropositive and 97 patients were syphilis-seronegative. In each sample, PCR tests for polA (TP0105), tmpC (TP0319), TP0136, TP0548 and 23S rRNA genes were performed. Samples taken from 91 patients were PCR-positive. Molecular typing of treponemal DNA wasbased on the sequencing of TP0136, TP0548 and 23S rRNA genes. Treponemal DNA was typeable in samples taken from 64 PCR-positive patients and 9 different genotypes were found. The proportion of treponemal strains resistant to macrolide antibiotics was 37,3%. In the DNA samples taken from 39 patients, a parallel treponemal typing approved by Centers for Disease Control and Prevention was performed. The variants of arp and tpr genes appear to combine independently with sequence variants of

Název v anglickém jazyce

Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genogypes are Related to the Sequence of the SS14 Strain

Popis výsledku anglicky

A set of 415 clinical samples isolated from 294 patients suspected of having syphilis collected in the Czech Republic between 2004 and 2010 was tested for the presence of treponemal DNA. Standard serological tests showed that 197 patients were syphilis-seropositive and 97 patients were syphilis-seronegative. In each sample, PCR tests for polA (TP0105), tmpC (TP0319), TP0136, TP0548 and 23S rRNA genes were performed. Samples taken from 91 patients were PCR-positive. Molecular typing of treponemal DNA wasbased on the sequencing of TP0136, TP0548 and 23S rRNA genes. Treponemal DNA was typeable in samples taken from 64 PCR-positive patients and 9 different genotypes were found. The proportion of treponemal strains resistant to macrolide antibiotics was 37,3%. In the DNA samples taken from 39 patients, a parallel treponemal typing approved by Centers for Disease Control and Prevention was performed. The variants of arp and tpr genes appear to combine independently with sequence variants of

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FE - Ostatní obory vnitřního lékařství

OECD FORD obor

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Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Dermato-Venereologica

ISSN

0001-5555

e-ISSN

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Svazek periodika

92

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

SE - Švédské království

Počet stran výsledku

6

Strana od-do

669-674

Kód UT WoS článku

000310828300030

EID výsledku v databázi Scopus

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