In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F11%3A00052566" target="_blank" >RIV/00216224:14110/11:00052566 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/11:00370061 RIV/68378050:_____/11:00370061

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10571-011-9669-2" target="_blank" >http://dx.doi.org/10.1007/s10571-011-9669-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10571-011-9669-2" target="_blank" >10.1007/s10571-011-9669-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain

Popis výsledku v původním jazyce

Pluripotent embryonic stem cells (ESCs) are able to differentiate into all cell types in the organism including cortical neurons. To follow the dynamic generation of progenitors of the dorsal forebrain in vitro, we generated ESCs from D6-GFP mice in which GFP marks neocortical progenitors and neurons after embryonic day (E) 10.5. We used several cell culture protocols for differentiation of ESCs into progenitors and neurons of the dorsal forebrain. In cell culture, GFP-positive cells were induced underdifferentiation conditions in quickly formed embryoid bodies (qEBs) after 10?12 day incubation. Activation of Wnt signaling during ESC differentiation further stimulated generation of D6-GFP-positive cortical cells. In contrast, differentiation protocolsusing normal embryoid bodies (nEBs) yielded only a few D6-GFPpositive cells. Gene expression analysis revealed that multiple components of the canonical Wnt signaling pathway were expressed during the development of embryoid bodies.

Název v anglickém jazyce

In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain

Popis výsledku anglicky

Pluripotent embryonic stem cells (ESCs) are able to differentiate into all cell types in the organism including cortical neurons. To follow the dynamic generation of progenitors of the dorsal forebrain in vitro, we generated ESCs from D6-GFP mice in which GFP marks neocortical progenitors and neurons after embryonic day (E) 10.5. We used several cell culture protocols for differentiation of ESCs into progenitors and neurons of the dorsal forebrain. In cell culture, GFP-positive cells were induced underdifferentiation conditions in quickly formed embryoid bodies (qEBs) after 10?12 day incubation. Activation of Wnt signaling during ESC differentiation further stimulated generation of D6-GFP-positive cortical cells. In contrast, differentiation protocolsusing normal embryoid bodies (nEBs) yielded only a few D6-GFPpositive cells. Gene expression analysis revealed that multiple components of the canonical Wnt signaling pathway were expressed during the development of embryoid bodies.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

<a href="/cs/project/GA204%2F08%2F1618" target="_blank" >GA204/08/1618: Molekulární podstata kanonické signální dráhy Wnt v embryonálním vývoji oka a mozku</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cellular and Molecular Neurobiology

ISSN

0272-4340

e-ISSN

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Svazek periodika

31

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

715-727

Kód UT WoS článku

000290914300008

EID výsledku v databázi Scopus

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