Buněčné vlastnosti kortikálních progenitorů se v kultuře nervových kmenových buněk nezachovávají

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F05%3A00023030" target="_blank" >RIV/68378050:_____/05:00023030 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

The cellular fate of cortical progenitors is not maintained in neurosphere cultures

Popis výsledku v původním jazyce

Neural progenitors of mouse forebrain can be propagated in vitro with bFGF and EGF as neurospheres. Less is clear if regional or developmental stage properties of these cells are kept in neurosphere cultures. We show that their original cell fate is lost. We isolated neural progenitors from dorsal telencephalon of D6-GFP mice for in vitro culture. The expression profile was specifically changed in cultured cells in 3 passages. Markers of the dorsal forebrain were downregulated and several ventrally-expressed genes were induced. Altered gene expression led to deep phenotypic change of cultured cells. D6-GFP+ cortical progenitors produce excitatory neurons in the cortex and few astrocytes in vivo but after culture in vitro they differentiate into many astrocytes, and oligodendrocytes and inhibitory neurons. Wnt signaling in cultured neurospheres was downregulated similarly as other dorsal markers but dominant active Wnt signaling retarded the loss of the dorsal identity in neurospheres.

Název v anglickém jazyce

The cellular fate of cortical progenitors is not maintained in neurosphere cultures

Popis výsledku anglicky

Neural progenitors of mouse forebrain can be propagated in vitro with bFGF and EGF as neurospheres. Less is clear if regional or developmental stage properties of these cells are kept in neurosphere cultures. We show that their original cell fate is lost. We isolated neural progenitors from dorsal telencephalon of D6-GFP mice for in vitro culture. The expression profile was specifically changed in cultured cells in 3 passages. Markers of the dorsal forebrain were downregulated and several ventrally-expressed genes were induced. Altered gene expression led to deep phenotypic change of cultured cells. D6-GFP+ cortical progenitors produce excitatory neurons in the cortex and few astrocytes in vivo but after culture in vitro they differentiate into many astrocytes, and oligodendrocytes and inhibitory neurons. Wnt signaling in cultured neurospheres was downregulated similarly as other dorsal markers but dominant active Wnt signaling retarded the loss of the dorsal identity in neurospheres.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Cellular Neuroscience

ISSN

1044-7431

e-ISSN

—

Svazek periodika

30

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

388-397

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—