Assesment of intracellular homocysteine concentration in sperms
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F11%3A00053108" target="_blank" >RIV/00216224:14110/11:00053108 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Assesment of intracellular homocysteine concentration in sperms
Popis výsledku v původním jazyce
The aim of this study was to evolve an effective procedure for the sperm lysis, obtention of intracellular content and subsequent assesment of Hc concentration and, in consequence, determine the correlations between blood plasma and seminal fluid Hc concentration, spermiogram parameters and DNA injury. Firstly, we used the method of repeated freezing and melting according to Ebisch et al., 2006 for the sperm lysis. Because the microscopic control of the samples didn?t show any markable sperm injury, weprojected and compared other methods of the cell lysis ? ultrasound, melting in ultrasound and freezing 10 times, methanol, distilled water, Triton X-100 solution, and lysis solution + 1% Triton X-100 solution. We used the method of high-performance liquid chromatography with fluorescence detection (HPLCFD) for the determination of Hc concentration and comet analysis for the assesment of DNA injury.
Název v anglickém jazyce
Assesment of intracellular homocysteine concentration in sperms
Popis výsledku anglicky
The aim of this study was to evolve an effective procedure for the sperm lysis, obtention of intracellular content and subsequent assesment of Hc concentration and, in consequence, determine the correlations between blood plasma and seminal fluid Hc concentration, spermiogram parameters and DNA injury. Firstly, we used the method of repeated freezing and melting according to Ebisch et al., 2006 for the sperm lysis. Because the microscopic control of the samples didn?t show any markable sperm injury, weprojected and compared other methods of the cell lysis ? ultrasound, melting in ultrasound and freezing 10 times, methanol, distilled water, Triton X-100 solution, and lysis solution + 1% Triton X-100 solution. We used the method of high-performance liquid chromatography with fluorescence detection (HPLCFD) for the determination of Hc concentration and comet analysis for the assesment of DNA injury.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
FK - Gynekologie a porodnictví
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů