The relationship between MSX1 gene mutation and tooth agenesis in the Czech population
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F13%3A00065637" target="_blank" >RIV/00216224:14110/13:00065637 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.sciencedirect.com/science/article/pii/S0901502713010278" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0901502713010278</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijom.2013.07.734" target="_blank" >10.1016/j.ijom.2013.07.734</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The relationship between MSX1 gene mutation and tooth agenesis in the Czech population
Popis výsledku v původním jazyce
Tooth development (odontogenesis) is a very complicated and complex process involving interplay between oral epithelium and adjacent ectomesenchyme. These reciprocal interactions are mediated by more than 300 signaling molecules (e.g. PAX9, AXIN2 and MSX1). Mutations in the genes for these two regulation proteins were associated with isolated (non-syndromic) form of hypodontia and for this reason; we decided to study gene mutations for MSX1 gene. Upon receipt of approval from the Ethical Commission of University Hospital Ostrava (No. 638/2011), DNA was isolated from patients using Ultra Clean Blood Spin DNA Isolation Kit (Mo-Bio). Blood samples or buccal swabs from 200 Czech patients with various types of tooth agenesis were used for this purpose. PCRproducts for exons of MSX1 gene were prepared using Kapa 2G Robust Hot Start polymerase (KapaBio Systems) and sequenced using ABI 3130 Prism (Life Technologies) genetic analyzer.
Název v anglickém jazyce
The relationship between MSX1 gene mutation and tooth agenesis in the Czech population
Popis výsledku anglicky
Tooth development (odontogenesis) is a very complicated and complex process involving interplay between oral epithelium and adjacent ectomesenchyme. These reciprocal interactions are mediated by more than 300 signaling molecules (e.g. PAX9, AXIN2 and MSX1). Mutations in the genes for these two regulation proteins were associated with isolated (non-syndromic) form of hypodontia and for this reason; we decided to study gene mutations for MSX1 gene. Upon receipt of approval from the Ethical Commission of University Hospital Ostrava (No. 638/2011), DNA was isolated from patients using Ultra Clean Blood Spin DNA Isolation Kit (Mo-Bio). Blood samples or buccal swabs from 200 Czech patients with various types of tooth agenesis were used for this purpose. PCRproducts for exons of MSX1 gene were prepared using Kapa 2G Robust Hot Start polymerase (KapaBio Systems) and sequenced using ABI 3130 Prism (Life Technologies) genetic analyzer.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
FF - ORL, oftalmologie, stomatologie
OECD FORD obor
—
Návaznosti výsledku
Projekt
<a href="/cs/project/NT11420" target="_blank" >NT11420: Molekulární diagnostika hypodoncie a možnosti zubních autotransplantací</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů