Intra- and Interlaboratory variability of paroxysmal nocturnal hemoglobinuria testing by flow cytometry following the 2012 practical guidelines for high-sensitivity paroxysmal nocturnal hemoglobinuria testing

Kód výsledku v IS VaVaI

RIV/00216224:14110/13:00070875 - isvavai.cz

Nalezeny alternativní kódy

RIV/00216208:11140/13:10135325 RIV/65269705:_____/13:#0002212 RIV/00669806:_____/13:10135325 RIV/00023736:_____/13:00010654

Výsledek na webu

http://dx.doi.org/10.1002/cyto.b.21075

DOI - Digital Object Identifier

10.1002/cyto.b.21075

Jazyk výsledku

angličtina

Název v původním jazyce

Intra- and Interlaboratory variability of paroxysmal nocturnal hemoglobinuria testing by flow cytometry following the 2012 practical guidelines for high-sensitivity paroxysmal nocturnal hemoglobinuria testing

Popis výsledku v původním jazyce

Sutherland et al. recently published the Practical Guidelines for high-sensitivity detection of paroxysmal nocturnal hemoglobinuria (PNH) clones by flow cytometry (FCM), containing concise protocols for PNH testing. METHODS: Using this approach, we studied the intra- and interlaboratory variability observed in a multicenter study in which fresh blood samples containing three clinically relevant PNH clone sizes within the granulocytic, monocytic, and red blood cell (RBC) populations were shipped to eachparticipating center. RESULTS: Coefficients of variation (CVs) for precision/reproducibility analysis ranged from 0.01%/0.02% to 0.48%/0.45% (big clone), from 0.69%/1.52% to 4.24%/5.80% (small-intermediate clone), from 1.47%/3.91% to 15.01% /17.83% (minor clone) for PNH white blood cells (WBCs) and from 0.24%/0.48% to 1.76%/1.83% (big clone), from 0.80%/1.14% to 2.39%/4.45% (small-intermediate clone), from 1.09%/3.36% to 10.54%/10.23% (minor clone) for PNH RBCs, respectively.

Název v anglickém jazyce

Intra- and Interlaboratory variability of paroxysmal nocturnal hemoglobinuria testing by flow cytometry following the 2012 practical guidelines for high-sensitivity paroxysmal nocturnal hemoglobinuria testing

Popis výsledku anglicky

Sutherland et al. recently published the Practical Guidelines for high-sensitivity detection of paroxysmal nocturnal hemoglobinuria (PNH) clones by flow cytometry (FCM), containing concise protocols for PNH testing. METHODS: Using this approach, we studied the intra- and interlaboratory variability observed in a multicenter study in which fresh blood samples containing three clinically relevant PNH clone sizes within the granulocytic, monocytic, and red blood cell (RBC) populations were shipped to eachparticipating center. RESULTS: Coefficients of variation (CVs) for precision/reproducibility analysis ranged from 0.01%/0.02% to 0.48%/0.45% (big clone), from 0.69%/1.52% to 4.24%/5.80% (small-intermediate clone), from 1.47%/3.91% to 15.01% /17.83% (minor clone) for PNH white blood cells (WBCs) and from 0.24%/0.48% to 1.76%/1.83% (big clone), from 0.80%/1.14% to 2.39%/4.45% (small-intermediate clone), from 1.09%/3.36% to 10.54%/10.23% (minor clone) for PNH RBCs, respectively.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytometry Part B-Clinical Cytometry

ISSN

1552-4949

e-ISSN

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Svazek periodika

84B

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

229-236

Kód UT WoS článku

000320732900005

EID výsledku v databázi Scopus

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