Flow Cytometry-Based Enumeration and Functional Characterization of CD8 T Regulatory Cells in Patients with Multiple Myeloma Before and After Lenalidomide Plus Dexamethasone Treatment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F14%3A00073417" target="_blank" >RIV/00216224:14110/14:00073417 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/65269705:_____/14:00061544

Výsledek na webu

<a href="http://dx.doi.org/10.1002/cyto.b.21109" target="_blank" >http://dx.doi.org/10.1002/cyto.b.21109</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cyto.b.21109" target="_blank" >10.1002/cyto.b.21109</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Flow Cytometry-Based Enumeration and Functional Characterization of CD8 T Regulatory Cells in Patients with Multiple Myeloma Before and After Lenalidomide Plus Dexamethasone Treatment

Popis výsledku v původním jazyce

Background: Multiple myeloma (MM) is a malignancy of plasma cells frequently associated with immune abnormalities. Several studies have confirmed that in MM immune deregulation can be mediated by increased numbers of CD4 T regulatory (Treg) cells, and these cells were also associated with poor outcome. In this study, we aimed to study CD8 Treg cells before and after lenalidomide plus dexamethasone (len-dex) treatment in MM patients. Methods: Using flow cytometry, we enumerated and assessed suppressive function of CD8 Treg cells in 16 MM patients before and after len-dex treatment. Results: Numbers of CD8 Treg cells (CD8+CD25hi+FoxP3+) (P &lt; 0.01) were significantly increased in MM patients (before treatment) compared to healthy donors. However, no significant changes were observed in CD4 and CD8 T cells. A significant increase in CD8 Treg cells was observed after len-dex treatment compared to pre-treatment but no significant difference was observed in CD4 and CD8 T cells.

Název v anglickém jazyce

Flow Cytometry-Based Enumeration and Functional Characterization of CD8 T Regulatory Cells in Patients with Multiple Myeloma Before and After Lenalidomide Plus Dexamethasone Treatment

Popis výsledku anglicky

Background: Multiple myeloma (MM) is a malignancy of plasma cells frequently associated with immune abnormalities. Several studies have confirmed that in MM immune deregulation can be mediated by increased numbers of CD4 T regulatory (Treg) cells, and these cells were also associated with poor outcome. In this study, we aimed to study CD8 Treg cells before and after lenalidomide plus dexamethasone (len-dex) treatment in MM patients. Methods: Using flow cytometry, we enumerated and assessed suppressive function of CD8 Treg cells in 16 MM patients before and after len-dex treatment. Results: Numbers of CD8 Treg cells (CD8+CD25hi+FoxP3+) (P &lt; 0.01) were significantly increased in MM patients (before treatment) compared to healthy donors. However, no significant changes were observed in CD4 and CD8 T cells. A significant increase in CD8 Treg cells was observed after len-dex treatment compared to pre-treatment but no significant difference was observed in CD4 and CD8 T cells.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytometry Part B ( Clinical cytometry)

ISSN

1552-4949

e-ISSN

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Svazek periodika

86

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

220-228

Kód UT WoS článku

000340544300001

EID výsledku v databázi Scopus

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