Functionally suppressive CD8 T regulatory cells are increased in patients with multiple myeloma: a cause for immune impairment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F12%3A00057824" target="_blank" >RIV/00216224:14110/12:00057824 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/65269705:_____/12:#0001882

Výsledek na webu

<a href="http://dx.doi.org/10.1371/journal.pone.0049446" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0049446</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0049446" target="_blank" >10.1371/journal.pone.0049446</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Functionally suppressive CD8 T regulatory cells are increased in patients with multiple myeloma: a cause for immune impairment

Popis výsledku v původním jazyce

Multiple myeloma (MM) is a plasma cell malignancy frequently associated with impaired immune cell numbers and functions. In MM, several studies have previously shown that CD4 regulatory T (Treg) cells hamper effector T cell functions and enhance immune dysfunction. In this study, we aimed to prove the presence of functionally suppressive Treg cells expressing CD8 phenotype (CD8 Treg cells) in MM. To the best of our knowledge, this has not been reported previously in MM. METHODS: We analyzed CD8 Treg cells and their transcription factor FoxP3 from 64 newly diagnosed MM patients using flow cytometry and real time-polymerase chain reaction (RT-PCR). RNA profile of cytokines in CD8 Treg cells was also assessed using RT-PCR. CD8 Treg cells from 5 MM patients and 5 healthy donors were functionally evaluated using proliferation assays. RESULTS: CD8 Treg cells (CD8+CD25hi+) were significantly elevated in MM patients (P&lt;0.0001), and their transcription factor FoxP3 expression was also higher

Název v anglickém jazyce

Functionally suppressive CD8 T regulatory cells are increased in patients with multiple myeloma: a cause for immune impairment

Popis výsledku anglicky

Multiple myeloma (MM) is a plasma cell malignancy frequently associated with impaired immune cell numbers and functions. In MM, several studies have previously shown that CD4 regulatory T (Treg) cells hamper effector T cell functions and enhance immune dysfunction. In this study, we aimed to prove the presence of functionally suppressive Treg cells expressing CD8 phenotype (CD8 Treg cells) in MM. To the best of our knowledge, this has not been reported previously in MM. METHODS: We analyzed CD8 Treg cells and their transcription factor FoxP3 from 64 newly diagnosed MM patients using flow cytometry and real time-polymerase chain reaction (RT-PCR). RNA profile of cytokines in CD8 Treg cells was also assessed using RT-PCR. CD8 Treg cells from 5 MM patients and 5 healthy donors were functionally evaluated using proliferation assays. RESULTS: CD8 Treg cells (CD8+CD25hi+) were significantly elevated in MM patients (P&lt;0.0001), and their transcription factor FoxP3 expression was also higher

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Public Library of Science one

ISSN

1932-6203

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

"e49446"

Kód UT WoS článku

000311234600041

EID výsledku v databázi Scopus

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