A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F14%3A00074018" target="_blank" >RIV/00216224:14110/14:00074018 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/14:00061210

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.celrep.2014.08.054" target="_blank" >http://dx.doi.org/10.1016/j.celrep.2014.08.054</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.celrep.2014.08.054" target="_blank" >10.1016/j.celrep.2014.08.054</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions

Popis výsledku v původním jazyce

DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes in recognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing a particular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects of Saw1 and its sumoylation suggest that Saw1 is a multifunctionalscaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.

Název v anglickém jazyce

A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions

Popis výsledku anglicky

DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes in recognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing a particular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects of Saw1 and its sumoylation suggest that Saw1 is a multifunctionalscaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell Reports

ISSN

2211-1247

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

143-152

Kód UT WoS článku

000344468100014

EID výsledku v databázi Scopus

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