Droplet digital PCR improves minimal residual disease monitoring in mature lymphoid tumors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F14%3A00076848" target="_blank" >RIV/00216224:14110/14:00076848 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Droplet digital PCR improves minimal residual disease monitoring in mature lymphoid tumors

Popis výsledku v původním jazyce

Minimal residual disease (MRD) detection allowed acquisition of valuable prognostic information in several mature lymphoid disorders with a considerable impact on clinical research (Ferrero S. 2011, Pott C. 2010). Although different methods can be used for MRD quantification, including flow cytometry (FC), real-time quantitative polymerase chain reaction (qPCR) remains the most validated and standardized method (Ladetto M. 2013, Pott C. 2011). However, despite remarkable sensitivity and specificity, qPCR has some drawbacks mainly related to the need for a reference standard curve, performed by target serial dilutions. The introduction of droplet digital PCR (ddPCR) has allowed to overcome this limitation, since its absolute quantification competence allows to avoid the need for a reference standard curve.

Název v anglickém jazyce

Droplet digital PCR improves minimal residual disease monitoring in mature lymphoid tumors

Popis výsledku anglicky

Minimal residual disease (MRD) detection allowed acquisition of valuable prognostic information in several mature lymphoid disorders with a considerable impact on clinical research (Ferrero S. 2011, Pott C. 2010). Although different methods can be used for MRD quantification, including flow cytometry (FC), real-time quantitative polymerase chain reaction (qPCR) remains the most validated and standardized method (Ladetto M. 2013, Pott C. 2011). However, despite remarkable sensitivity and specificity, qPCR has some drawbacks mainly related to the need for a reference standard curve, performed by target serial dilutions. The introduction of droplet digital PCR (ddPCR) has allowed to overcome this limitation, since its absolute quantification competence allows to avoid the need for a reference standard curve.

Druh

O - Ostatní výsledky

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů