SLOW SULFIDE DONOR GYY4137 DIFFERENTIATES NG108-15 NEURONAL CELLS THROUGH DIFFERENT INTRACELLULAR TRANSPORTERS THAN dbcAMP

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F16%3A00090728" target="_blank" >RIV/00216224:14110/16:00090728 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.neuroscience.2016.03.057" target="_blank" >http://dx.doi.org/10.1016/j.neuroscience.2016.03.057</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.neuroscience.2016.03.057" target="_blank" >10.1016/j.neuroscience.2016.03.057</a>

Jazyk výsledku

angličtina

Název v původním jazyce

SLOW SULFIDE DONOR GYY4137 DIFFERENTIATES NG108-15 NEURONAL CELLS THROUGH DIFFERENT INTRACELLULAR TRANSPORTERS THAN dbcAMP

Popis výsledku v původním jazyce

Cellular differentiation is the process, by which a cell changes from one cell type to another, preferentially to the more specialized one. Calcium fluxes play an important role in this action. Differentiated NG108-15 or PC12 cells serve as models for studying neuronal pathways. NG108-15 cell line is a reliable model of cholinergic neuronal cells. These cells differentiate to a neuronal phenotype due to the dibutyryl cAMP (dbcAMP) treatment. We have shown that a slow sulfide donor-GYY4137-can also act as a differentiating factor in NG108-15 cell line. Calcium is an unavoidable ion required in NG108-15 cell differentiation by both, dbcAMP and GYY4137, since cultivation in EGTA completely prevented differentiation of these cells. In this work we focused primarily on the role of reticular calcium in the process of NG108-15 cell differentiation. We have found that dbcAMP and also GYY4137 decreased reticular calcium concentration by different mechanisms.

Název v anglickém jazyce

SLOW SULFIDE DONOR GYY4137 DIFFERENTIATES NG108-15 NEURONAL CELLS THROUGH DIFFERENT INTRACELLULAR TRANSPORTERS THAN dbcAMP

Popis výsledku anglicky

Cellular differentiation is the process, by which a cell changes from one cell type to another, preferentially to the more specialized one. Calcium fluxes play an important role in this action. Differentiated NG108-15 or PC12 cells serve as models for studying neuronal pathways. NG108-15 cell line is a reliable model of cholinergic neuronal cells. These cells differentiate to a neuronal phenotype due to the dibutyryl cAMP (dbcAMP) treatment. We have shown that a slow sulfide donor-GYY4137-can also act as a differentiating factor in NG108-15 cell line. Calcium is an unavoidable ion required in NG108-15 cell differentiation by both, dbcAMP and GYY4137, since cultivation in EGTA completely prevented differentiation of these cells. In this work we focused primarily on the role of reticular calcium in the process of NG108-15 cell differentiation. We have found that dbcAMP and also GYY4137 decreased reticular calcium concentration by different mechanisms.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

NEUROSCIENCE

ISSN

0306-4522

e-ISSN

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Svazek periodika

325

Číslo periodika v rámci svazku

"JUN 14 2016"

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

100-110

Kód UT WoS článku

000374503200010

EID výsledku v databázi Scopus

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