Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F17%3A00095147" target="_blank" >RIV/00216224:14110/17:00095147 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_11" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-7021-6_11</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_11" target="_blank" >10.1007/978-1-4939-7021-6_11</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells

Popis výsledku v původním jazyce

Lung epithelium contains distinctive subpopulations of lung stem/progenitor cells (LSPCs) that are essential for lung epithelial maintenance and repair in vivo. Hence, LSPCs are in the center of interest of lung biology due to their promising therapeutic applications. To reach this goal, proper characterization of LSPCs, understanding of their proliferation and differentiation potentials and elucidation of mechanisms that control them are necessary. Therefore, development of reliable in vitro clonogenic assays has been needed. We established lungosphere assay, an in vitro sphere-forming 3D culture assay that enables to evaluate stem/progenitor cell activity, self-renewal and differentiation capacity of LSPCs and to conveniently test the effect of various treatments on LSPCs. Here we provide a detailed description of procedures for isolation of adult mouse lung epithelial cells, their culture in non-adherent conditions to form LSPC-derived spheroids (lungospheres) and for embedding of lungospheres into 3D extracellular matrix to model processes of lung tissue maintenance in a physiologically relevant microenvironment.

Název v anglickém jazyce

Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells

Popis výsledku anglicky

Lung epithelium contains distinctive subpopulations of lung stem/progenitor cells (LSPCs) that are essential for lung epithelial maintenance and repair in vivo. Hence, LSPCs are in the center of interest of lung biology due to their promising therapeutic applications. To reach this goal, proper characterization of LSPCs, understanding of their proliferation and differentiation potentials and elucidation of mechanisms that control them are necessary. Therefore, development of reliable in vitro clonogenic assays has been needed. We established lungosphere assay, an in vitro sphere-forming 3D culture assay that enables to evaluate stem/progenitor cell activity, self-renewal and differentiation capacity of LSPCs and to conveniently test the effect of various treatments on LSPCs. Here we provide a detailed description of procedures for isolation of adult mouse lung epithelial cells, their culture in non-adherent conditions to form LSPC-derived spheroids (lungospheres) and for embedding of lungospheres into 3D extracellular matrix to model processes of lung tissue maintenance in a physiologically relevant microenvironment.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

3D Cell Culture : Methods and Protocols

ISBN

9781493970193

ISSN

1064-3745

e-ISSN

—

Počet stran výsledku

17

Strana od-do

149-165

Název nakladatele

Humana Press

Místo vydání

New York

Místo konání akce

New York

Datum konání akce

1. 1. 2017

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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